Increased Expression of Anaphylatoxin C5a-Receptor-1 in Neutrophils and Natural Killer Cells of Preterm Infants
Abstract
:1. Introduction
2. Results
2.1. Cohort Characteristics
2.2. Flow Cytometry
2.2.1. Elevated Intracellular Expression of C5aR1 in Preterm Neutrophils
2.2.2. Density of C5aR1 Expression in NK Cells of Preterm Infants and on Their Surface Is Elevated
2.2.3. Higher C5aR2 Expression Neutrophils of Preterm and Term Infants as Compared to Healthy Adults
3. Discussion
4. Materials and Methods
4.1. Study Cohort
4.2. Ethics
4.3. Flow Cytometry
4.4. Clinical Data
4.5. Statistical Analysis
Data Analysis Was Performed Using GraphPad Prism®®
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Preterm infants (n) | 32 |
---|---|
Gestational age, weeks of gestation (mean/median/SD) | 29.5/29.9/2.69 |
Birth weight, g (mean/median/SD) | 1331.6/1300.0/464.13 |
Gender male (n/%) | 14/43.8 |
Multiples (n/%) | 10/31.3 |
AIS (n/%) | 8/25.0 |
EOS (n/%) | 1/3.1 |
Mothers (n) | 25 |
Age, years (mean/median/SD) | 29.0/29.0/6.09 |
Delivery at gestational age, weeks of gestation (mean/median/SD) | 29.7/29.9/2.68 |
AIS yes (n/%) | 6/24.0 |
Term infants (n) | 11 |
Sex, male (n/%) | 4/36.4 |
Adults (n) | 14 |
Sex, male (n/%) | 6/42.9 |
Age, years (mean/median/SD) | 28.2/25.0/5.96 |
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Boeckel, H.; Karsten, C.M.; Göpel, W.; Herting, E.; Rupp, J.; Härtel, C.; Hartz, A. Increased Expression of Anaphylatoxin C5a-Receptor-1 in Neutrophils and Natural Killer Cells of Preterm Infants. Int. J. Mol. Sci. 2023, 24, 10321. https://doi.org/10.3390/ijms241210321
Boeckel H, Karsten CM, Göpel W, Herting E, Rupp J, Härtel C, Hartz A. Increased Expression of Anaphylatoxin C5a-Receptor-1 in Neutrophils and Natural Killer Cells of Preterm Infants. International Journal of Molecular Sciences. 2023; 24(12):10321. https://doi.org/10.3390/ijms241210321
Chicago/Turabian StyleBoeckel, Hannah, Christian M. Karsten, Wolfgang Göpel, Egbert Herting, Jan Rupp, Christoph Härtel, and Annika Hartz. 2023. "Increased Expression of Anaphylatoxin C5a-Receptor-1 in Neutrophils and Natural Killer Cells of Preterm Infants" International Journal of Molecular Sciences 24, no. 12: 10321. https://doi.org/10.3390/ijms241210321