Anti-Fibrotic Effects of RF Electric Currents
Abstract
:1. Introduction
2. Results
2.1. Extracellular Matrix Production
2.2. Cell Proliferation and Death
2.3. Cell Migration
2.4. Expression of Metalloproteinases MMP1 and MMP9
2.5. Expression and Activation of MAP-Kinase ERK1/2 and Nuclear Factor (NF)-Kappa B p65
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Electric Treatment
4.3. XTT Proliferation Assay
4.4. TUNEL Assay
4.5. Wound Assay
4.6. Immunofluorescence for α-SMA, Collagen Type I and Collagen Type III
4.7. Immunoblot for α-SMA, MMP1, MMP9, ERK, P-ERK, NF-κB and p-NF-κB
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hernández-Bule, M.L.; Toledano-Macías, E.; Pérez-González, L.A.; Martínez-Pascual, M.A.; Fernández-Guarino, M. Anti-Fibrotic Effects of RF Electric Currents. Int. J. Mol. Sci. 2023, 24, 10986. https://doi.org/10.3390/ijms241310986
Hernández-Bule ML, Toledano-Macías E, Pérez-González LA, Martínez-Pascual MA, Fernández-Guarino M. Anti-Fibrotic Effects of RF Electric Currents. International Journal of Molecular Sciences. 2023; 24(13):10986. https://doi.org/10.3390/ijms241310986
Chicago/Turabian StyleHernández-Bule, María Luisa, Elena Toledano-Macías, Luis Alfonso Pérez-González, María Antonia Martínez-Pascual, and Montserrat Fernández-Guarino. 2023. "Anti-Fibrotic Effects of RF Electric Currents" International Journal of Molecular Sciences 24, no. 13: 10986. https://doi.org/10.3390/ijms241310986