Changes of the Protein CoAlation Pattern in Response to Oxidative Stress and Capacitation in Human Spermatozoa
Abstract
:1. Introduction
2. Results
2.1. Protein CoAlation Pattern Differentially Changed during In Vitro Induced Oxidative Stress in Human Spermatozoa
2.2. The Inhibition of 2-Cys Peroxiredoxins Increases the Level of Protein CoAlation in Spermatozoa
2.3. Protein CoAlation Pattern during Time-Course Sperm Capacitation
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Sperm Sample Preparation
4.3. Induction of In Vitro Oxidative Stress by Hydrogen Peroxide, Diamide and Tert-Butyl Hydroperoxide
4.4. Inhibition of PRDXs in Spermatozoa Treated with H2O2 to Promote In Vitro Oxidative Stress
4.5. Sperm Viability and Motility Analysis
4.6. Profile of Protein CoAlation during Time-Course Sperm Capacitation
4.7. SDS PAGE and Immunoblotting
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Source of Variation | Protein CoAlation Levels | Sperm Viability | Total Motility | Progressive Motility |
---|---|---|---|---|
H2O2 treatment | p < 0.001 | p = 0.002 | p < 0.0001 | p < 0.0001 |
time | p = 0.0007 | p = 0.03 | p = 0.20 | p = 0.601 |
H2O2 treatment × time | p = 0.077 | p = 0.014 | p = 0.83 | p = 0.99 |
Diamide treatment | p < 0.0001 | p = 0.003 | p < 0.0001 | p < 0.0001 |
time | p < 0.0001 | p = 0.04 | p = 0.60 | p = 0.47 |
Diamide treatment × time | p = 0.0151 | p = 0.06 | p = 0.45 | p = 0.57 |
t-BHP treatment | p < 0.0001 | p = 0.49 | p < 0.0001 | p < 0.0001 |
time | p < 0.0001 | p = 0.45 | p = 0.61 | p < 0.001 |
t-BHP treatment × time | p = 0.0006 | p = 0.29 | p = 0.24 | p = 0.89 |
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Petrone, O.; Serafini, S.; Yu, B.Y.K.; Filonenko, V.; Gout, I.; O’Flaherty, C. Changes of the Protein CoAlation Pattern in Response to Oxidative Stress and Capacitation in Human Spermatozoa. Int. J. Mol. Sci. 2023, 24, 12526. https://doi.org/10.3390/ijms241512526
Petrone O, Serafini S, Yu BYK, Filonenko V, Gout I, O’Flaherty C. Changes of the Protein CoAlation Pattern in Response to Oxidative Stress and Capacitation in Human Spermatozoa. International Journal of Molecular Sciences. 2023; 24(15):12526. https://doi.org/10.3390/ijms241512526
Chicago/Turabian StylePetrone, Olivia, Steven Serafini, Bess Yi Kun Yu, Valeriy Filonenko, Ivan Gout, and Cristian O’Flaherty. 2023. "Changes of the Protein CoAlation Pattern in Response to Oxidative Stress and Capacitation in Human Spermatozoa" International Journal of Molecular Sciences 24, no. 15: 12526. https://doi.org/10.3390/ijms241512526