Alteration of Mitochondrial Transcript Expression in Arabidopsis thaliana Using a Custom-Made Library of Pentatricopeptide Repeat Proteins
Abstract
:1. Introduction
2. Results
2.1. Creating a Library of RPF2 Proteins
2.2. Genotype and Phenotype Analysis
2.3. Analysis of the Mitochondrial Respiratory Complexes
2.4. Further Characterisation of Transformant #280
3. Discussion
4. Materials and Methods
4.1. Plant Growth
4.2. Construction of the PPR Library
4.3. Genotypic Analysis
4.4. Phenotypic Analysis
4.5. High-Throughput BN-PAGE and Western Blotting
4.6. RNA Extraction and qPCR
4.7. Proteomics Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Vincis Pereira Sanglard, L.; Small, I.D.; Colas des Francs-Small, C. Alteration of Mitochondrial Transcript Expression in Arabidopsis thaliana Using a Custom-Made Library of Pentatricopeptide Repeat Proteins. Int. J. Mol. Sci. 2023, 24, 13233. https://doi.org/10.3390/ijms241713233
Vincis Pereira Sanglard L, Small ID, Colas des Francs-Small C. Alteration of Mitochondrial Transcript Expression in Arabidopsis thaliana Using a Custom-Made Library of Pentatricopeptide Repeat Proteins. International Journal of Molecular Sciences. 2023; 24(17):13233. https://doi.org/10.3390/ijms241713233
Chicago/Turabian StyleVincis Pereira Sanglard, Lilian, Ian D. Small, and Catherine Colas des Francs-Small. 2023. "Alteration of Mitochondrial Transcript Expression in Arabidopsis thaliana Using a Custom-Made Library of Pentatricopeptide Repeat Proteins" International Journal of Molecular Sciences 24, no. 17: 13233. https://doi.org/10.3390/ijms241713233