ALK, ROS1, RET and NTRK1–3 Gene Fusions in Colorectal and Non-Colorectal Microsatellite-Unstable Cancers
Abstract
:1. Introduction
2. Results
2.1. MSI Detection
2.2. Analysis of Gene Rearrangements
2.3. Pattern of Gene Rearrangement in Various Categories of Microsatellite-Unstable Tumors
2.4. MSI Analysis in Non-Colorectal Tumors Carrying ALK/ROS/RET/NTRK Rearrangements
3. Discussion
4. Materials and Methods
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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ID | Sex | Age, Years | Diagnosis | RAS/RAF Mutations | 5′/3′-End Expression Imbalance | Fusion | Method of Fusion Identification | |
---|---|---|---|---|---|---|---|---|
1. | A9365 | m | 74 | CRC | negative | ALK | STRN::ALK (S5;ins53A20) | NGS |
2. | C1182 | f | 71 | CRC | negative | ALK | EML4::ALK (E6;A20) | PCR |
3. | C3234 | f | 49 | CRC | negative | ALK | SPTBN1::ALK (S7;A20) | NGS |
4. | D0934 | m | 70 | CRC | negative | ALK | RBBP8::ALK (R8;A20) | NGS |
5. | P28580 | f | 66 | CRC | negative | ALK | SPTBN1::ALK (S7;A20) | NGS |
6. | P30811 | m | 80 | CRC | negative | ALK | EML4::ALK (E20;A20) | PCR |
7. | B9775 | f | 62 | CRC | negative | - | CCDC6::RET (C1;RE12) | PCR |
8. | C7622 | m | 58 | CRC | negative | - | CCDC6::RET (C1;RE12) | PCR |
9. | A7310 | m | 65 | CRC | negative | - | CCDC6::RET (C1;RE12) | PCR |
10. | P22395 | m | 62 | CRC | negative | - | CCDC6::RET (C1;RE12) | PCR |
11. | B8013 | f | 63 | CRC | negative | RET | NCOA4::RET (N8;R12) | PCR |
12. | C2542 | f | 67 | CRC | negative | RET | NCOA4::RET (N9del501;R12) | NGS |
13. | C4286 | m | 54 | CRC | negative | RET | NCOA4::RET (N9;R12) | NGS |
14. | P16667 | f | 65 | CRC | negative | RET | NCOA4::RET (N9;R12) | NGS |
15. | P24158 | f | 66 | CRC | negative | RET | CCDC6::RET (C1;RE12) | PCR |
16. | A4695 | f | 64 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
17. | B2240 | f | 60 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
18. | B9472 | m | 52 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
19. | C1589 | f | 64 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
20. | C6335 | f | 69 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
21. | C6755 | m | 72 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
22. | D0273 | m | 68 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
23. | D1407 | f | 58 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
24. | E2169 | m | 58 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
25. | P18891 | f | 59 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N12) | PCR |
26. | P21693 | m | 86 | CRC | negative | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
27. | E4114 | f | 69 | CRC | negative | NTRK1 | LMNA::NTRK1 (L3;N10) | PCR |
28. | B9540 | m | 72 | CRC | negative | NTRK1 | TPR::NTRK1 (T15;N10) | NGS |
29. | C4763 | m | 65 | CRC | negative | NTRK1 | TPR::NTRK1 (T20;N11) | NGS |
30. | C7112 | f | 65 | CRC | negative | NTRK1 | ZKSCAN1::NTRK1 (Z3;N12) | NGS |
31. | D0155 | f | 81 | CRC | negative | NTRK1 | LMNA::NTRK1 (L9ins44;del74N11) | NGS |
32. | B9665 | m | 74 | CRC | negative | NTRK2 | ETV6::NTRK2 (E5;N15) | NGS |
33. | A4890 | f | 65 | CRC | negative | NTRK3 | EML4::NTRK3 (E2;N14) | PCR |
34. | B1263 | f | 58 | CRC | negative | - | EML4::NTRK3 (E2;N14) | PCR |
35. | B4631 | f | 68 | CRC | negative | NTRK3 | EML4::NTRK3 (E2;N14) | PCR |
36. | C3968 | f | 70 | CRC | negative | NTRK3 | EML4::NTRK3 (E2;N14) | PCR |
37. | C5878 | m | 57 | CRC | negative | NTRK3 | ETV6::NTRK3 (E5;N15) | PCR |
38. | C6430 | f | 72 | CRC | negative | NTRK3 | EML4::NTRK3 (E2;N14) | PCR |
39. | C7912 | f | 54 | CRC | negative | NTRK3 | EML4::NTRK3 (E2;N14) | PCR |
40. | D7461 | f | 61 | CRC | negative | NTRK3 | ETV6::NTRK3 (E5;N15) | PCR |
41. | P22904 | f | 68 | CRC | negative | NTRK3 | EML4::NTRK3 (E2;N14) | PCR |
42. | P23972 | f | 67 | CRC | negative | NTRK3 | ETV6::NTRK3 (E5;N15) | PCR |
43. | B4561 | m | 31 | CRC | KRAS p.A146T | NTRK2 | ETV6::NTRK2 (E5;N15) | NGS |
44. | C1274 | f | 73 | CRC | BRAF p.V600E | - | ETV6::NTRK3 (E5;N15) | PCR |
45. | C3231 | f | 76 | CRC | BRAF p.V600E | NTRK1 | LMNA::NTRK1 (L3;N10) | PCR |
46. | P29082 | f | 85 | CRC | NRAS p.Q61K | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
47. | C7527 | f | 71 | GC | KRAS p.G12C | NTRK1 | TPM3::NTRK1 (T8;N10) | PCR |
48. | C4292 | f | 72 | CRC | Negative | ALK | not defined | n/a for NGS |
49. | C8576 | m | 66 | CRC | Negative | ALK | not defined | n/a for NGS |
50. | C2716 | m | 80 | CRC | Negative | RET | not defined | n/a for NGS |
51. | C5821 | m | 83 | CRC | Negative | RET | not defined | n/a for NGS |
52. | P15387 | m | 68 | CRC | Negative | RET | not defined | n/a for NGS |
53. | B9814 | f | 65 | CRC | Negative | NTRK1 | not defined | n/a for NGS |
54. | C4831 | f | 60 | CRC | Negative | NTRK1 | not defined | n/a for NGS |
55. | D0795 | m | 74 | CRC | Negative | NTRK1 | not defined | n/a for NGS |
56. | C5665 | f | 68 | CRC | Negative | NTRK3 | not defined | n/a for NGS |
57. | C9396 | m | 32 | CRC | Negative | NTRK3 | not defined | n/a for NGS |
58. | D3390 | f | 80 | CRC | Negative | NTRK3 | not defined | n/a for NGS |
59. | D5144 | m | 66 | CRC | KRAS p.G12D | NTRK3 | not defined | n/a for NGS |
60. | A9663 | m | 66 | GC | Negative | NTRK1 | not defined | n/a for NGS |
61. | C1642 | m | 83 | GC | Negative | NTRK1 | not defined | n/a for NGS |
62. | E3046 | m | 34 | GC | Negative | NTRK3 | not defined | n/a for NGS |
63. | C8708 | f | 67 | EC | Negative | NTRK3 | not defined | n/a for NGS |
64. | D5486 | f | 71 | EC | Negative | NTRK3 | not defined | n/a for NGS |
65. | D6096 | f | 64 | EC | Negative | NTRK3 | not defined | n/a for NGS |
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Mulkidjan, R.S.; Saitova, E.S.; Preobrazhenskaya, E.V.; Asadulaeva, K.A.; Bubnov, M.G.; Otradnova, E.A.; Terina, D.M.; Shulga, S.S.; Martynenko, D.E.; Semina, M.V.; et al. ALK, ROS1, RET and NTRK1–3 Gene Fusions in Colorectal and Non-Colorectal Microsatellite-Unstable Cancers. Int. J. Mol. Sci. 2023, 24, 13610. https://doi.org/10.3390/ijms241713610
Mulkidjan RS, Saitova ES, Preobrazhenskaya EV, Asadulaeva KA, Bubnov MG, Otradnova EA, Terina DM, Shulga SS, Martynenko DE, Semina MV, et al. ALK, ROS1, RET and NTRK1–3 Gene Fusions in Colorectal and Non-Colorectal Microsatellite-Unstable Cancers. International Journal of Molecular Sciences. 2023; 24(17):13610. https://doi.org/10.3390/ijms241713610
Chicago/Turabian StyleMulkidjan, Rimma S., Evgeniya S. Saitova, Elena V. Preobrazhenskaya, Karimat A. Asadulaeva, Mikhail G. Bubnov, Ekaterina A. Otradnova, Darya M. Terina, Sofia S. Shulga, Darya E. Martynenko, Maria V. Semina, and et al. 2023. "ALK, ROS1, RET and NTRK1–3 Gene Fusions in Colorectal and Non-Colorectal Microsatellite-Unstable Cancers" International Journal of Molecular Sciences 24, no. 17: 13610. https://doi.org/10.3390/ijms241713610