Method Development for Determination of Doripenem in Human Plasma via Capillary Electrophoresis Coupled with Field-Enhanced Sample Stacking and Sweeping
Abstract
:1. Introduction
2. Results and Discussion
2.1. Extraction Process
2.2. The Concept of FESS-Sweeping MEKC Model
2.3. Optimization of FESS-Sweeping MEKC Model
2.3.1. The pH Value of Background Solution
2.3.2. The Organic Modifier in HCB
2.3.3. The Phosphate Concentration in HCB and Sample Matrix
2.3.4. Time for Sample Injection and the Concentration of SDS
2.4. Method Validation
2.4.1. Linearity
2.4.2. Accuracy, Precision, and Recovery
2.4.3. Selectivity and Specificity
2.4.4. Limit of Detection (LOD), Limit of Quantification (LOQ), and Sensitivity Enhancement
2.5. Application
3. Materials and Methods
3.1. Materials and Chemicals
3.2. Pretreatment and Extraction of Plasma Samples
3.3. CE System
3.4. FESS-Sweeping MEKC Model
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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(A) Doripenem | (B) Meropenem | ||
---|---|---|---|
MW 1 = 438.52 pKa1 = 4.37; pKa2 = 9.39 | MW 1 = 383.46 pKa1 = 4.37; pKa2 = 8.34 |
Doripenem | Linearity (1–45 μg/mL) | Precision and Accuracy | |||
---|---|---|---|---|---|
Regression Equation | r2 | Spiked Conc. (μg/mL) | RSD (%) | RE (%) | |
Intra-day (n = 3) | y = (0.106 ± 0.0059) x + (0.0337 ± 0.0259) | 0.9996 | 5.00 25.00 40.00 | 1.29 1.20 1.35 | −3.01 3.33 2.05 |
Inter-day (n = 5) | y = (0.1087 ± 0.0037) x + (0.0394 ± 0.0394) | 0.9995 | 5.00 25.00 40.00 | 5.86 1.17 1.96 | −4.63 2.36 2.01 |
Patient | Daily Dose | Dosage Interval | Collection Time Point (h) | Concentration Ranges (Premedication/Post Administration, μg/mL) | ||||
---|---|---|---|---|---|---|---|---|
Continuous administration | ||||||||
P1 | 1.5 g | 1.5 g per day | 0 | 4.5 | 6 | 24 | 48 | 0/15.06–39.00 |
P2 | 1.5 g | 1.5 g per day | 0 | 3 | 6 | 24 | 48 | 0/11.39–37.53 |
Intermittent administration | ||||||||
P3 | 1.5 g | 0.5 g per 8 h | 0 | 1 | 2 | 6 | 8 | 0/9.37–44.97 |
P4 | 0.5 g | 0.25 g per 12 h | 0 | 0.75 | 2 | 4 | 8 | 0/9.64–44.86 |
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Liang, H.-H.; Lin, Y.-C.; Hung, C.-C.; Hou, Y.-C.; Lin, Y.-H. Method Development for Determination of Doripenem in Human Plasma via Capillary Electrophoresis Coupled with Field-Enhanced Sample Stacking and Sweeping. Int. J. Mol. Sci. 2023, 24, 13751. https://doi.org/10.3390/ijms241813751
Liang H-H, Lin Y-C, Hung C-C, Hou Y-C, Lin Y-H. Method Development for Determination of Doripenem in Human Plasma via Capillary Electrophoresis Coupled with Field-Enhanced Sample Stacking and Sweeping. International Journal of Molecular Sciences. 2023; 24(18):13751. https://doi.org/10.3390/ijms241813751
Chicago/Turabian StyleLiang, Hsin-Hua, Yu-Chao Lin, Chin-Chuan Hung, Yu-Chi Hou, and Yi-Hui Lin. 2023. "Method Development for Determination of Doripenem in Human Plasma via Capillary Electrophoresis Coupled with Field-Enhanced Sample Stacking and Sweeping" International Journal of Molecular Sciences 24, no. 18: 13751. https://doi.org/10.3390/ijms241813751