Can Pyomelanin Produced by Pseudomonas aeruginosa Promote the Regeneration of Gastric Epithelial Cells and Enhance Helicobacter pylori Phagocytosis?
Abstract
:1. Introduction
2. Results
2.1. Antibacterial Activity of PyoM towards H. pylori
2.2. PyoM Neutralizes the Cytotoxic Effect of H. pylori LPS towards Gastric Epithelial Cells and Monocytes
2.3. LPS-Induced Apoptosis Is Diminished in the Presence of PyoMsol
2.4. PyoM Neutralizes Reactive Oxygen Species Produced by Cells Exposed to H. pylori LPS
2.5. PyoMsol Promotes the Migration of Gastric Epithelial Cells Affected by H. pylori LPS
2.6. PyoMsol Enhances the Phagocytic Capacity of Monocytes towards Fluorescently Labelled E. coli or H. pylori
3. Discussion
4. Materials and Methods
4.1. Growth Conditions of Pseudomonas aeruginosa
4.2. Isolation and Purification of PyoMinsol and PyoMsol
4.3. Cell Cultures
4.4. Cell Stimulators
4.5. Antibacterial Activity of PyoMinsol and PyoMsol
4.6. Cell’s Viability Assay
4.7. Reactive Oxygen Species
4.8. Apoptosis
4.9. Wound Healing Assay
4.10. Phagocytosis
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Urbaniak, M.M.; Rudnicka, K.; Gościniak, G.; Chmiela, M. Can Pyomelanin Produced by Pseudomonas aeruginosa Promote the Regeneration of Gastric Epithelial Cells and Enhance Helicobacter pylori Phagocytosis? Int. J. Mol. Sci. 2023, 24, 13911. https://doi.org/10.3390/ijms241813911
Urbaniak MM, Rudnicka K, Gościniak G, Chmiela M. Can Pyomelanin Produced by Pseudomonas aeruginosa Promote the Regeneration of Gastric Epithelial Cells and Enhance Helicobacter pylori Phagocytosis? International Journal of Molecular Sciences. 2023; 24(18):13911. https://doi.org/10.3390/ijms241813911
Chicago/Turabian StyleUrbaniak, Mateusz M., Karolina Rudnicka, Grażyna Gościniak, and Magdalena Chmiela. 2023. "Can Pyomelanin Produced by Pseudomonas aeruginosa Promote the Regeneration of Gastric Epithelial Cells and Enhance Helicobacter pylori Phagocytosis?" International Journal of Molecular Sciences 24, no. 18: 13911. https://doi.org/10.3390/ijms241813911