Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions
Abstract
:1. Introduction
2. Results
2.1. Blood Pressure of Diabetic Patients and db/db Mice
2.2. Alteration of CSE Expression and H2S Levels in the Kidney Tissues of Diabetic Patients, db/db Mice and JG Cells
2.3. The Effect of Exogenous H2S on Renin Expression, Renin Activity and Renin Release
2.4. Exogenous H2S Inhibited RAAS Activation by Modulating the PDE3B-cAMP Pathway
2.5. Exogenous H2S Attenuated Renin Release via SNARE Proteins
2.6. Exogenous H2S Promoted Renin Consumption through Activation of Autophagy
3. Discussion
4. Materials and Methods
4.1. Study Participants
4.2. Animal Studies
4.3. Blood Pressure Measurement of Mice
4.4. Serum and Tissue Collection
4.5. Immunohistochemistry
4.6. Culture, Treatment and Transfection of JG Cells
4.7. Immunofluorescence Staining
4.8. Measurement of H2S Level
4.9. Western Blot Analysis
4.10. Measurement of Renin Activity and Content
4.11. cAMP Content Measurement
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, N.; Li, M.; Liu, S.; Kang, J.; Chen, L.; Huang, J.; Wang, Y.; Chen, H.; Zhang, W. Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions. Int. J. Mol. Sci. 2023, 24, 1690. https://doi.org/10.3390/ijms24021690
Liu N, Li M, Liu S, Kang J, Chen L, Huang J, Wang Y, Chen H, Zhang W. Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions. International Journal of Molecular Sciences. 2023; 24(2):1690. https://doi.org/10.3390/ijms24021690
Chicago/Turabian StyleLiu, Ning, Mingyu Li, Siyuan Liu, Jiaxin Kang, Lingxue Chen, Jiayi Huang, Yan Wang, He Chen, and Weihua Zhang. 2023. "Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions" International Journal of Molecular Sciences 24, no. 2: 1690. https://doi.org/10.3390/ijms24021690