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Article
Peer-Review Record

The Accumulation of Volatile Compounds and the Change in the Morphology of the Leaf Wax Cover Accompanied the “Anti-Aging” Effect in Anethum graveolens L. Plants Sprayed with 6-Benzylaminopurine

Int. J. Mol. Sci. 2023, 24(20), 15137; https://doi.org/10.3390/ijms242015137
by Anna V. Shirokova 1,*, Lev B. Dmitriev 2, Sergey L. Belopukhov 2, Valeria L. Dmitrieva 2, Irina L. Danilova 3, Viktor A. Kharchenko 4, Olga A. Pekhova 3, Elena F. Myagkih 3, Andrey N. Tsitsilin 5, Alexander A. Gulevich 6, Ekaterina V. Zhuravleva 7, Yulia N. Kostanchuk 3,* and Ekaterina N. Baranova 6,8
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2023, 24(20), 15137; https://doi.org/10.3390/ijms242015137
Submission received: 31 August 2023 / Revised: 27 September 2023 / Accepted: 28 September 2023 / Published: 13 October 2023

Round 1

Reviewer 1 Report

Overview and general recommendation:

Thanks for the opportunity to review this research. The manuscript entitled The Accumulation of Volatile Compounds and the Change in the Morphology of the Leaf Wax Cover Accompanied the "an-ti-aging" Effect in Anethum graveolens L. Plants Sprayed with 6-BAP” demonstrates the potential effect of exogenous cytokinin 6-BAP on the growth and development of Anethum graveolens plants. The subject of the manuscript is topical and the article is very interesting to read.

1.         Introduction

The introduction is well structured, but the authors should also highlight the importance of the study.

2.         Materials and Methods

The used methods are accurate.

3.         Results and discussion

The results are clearly presented. All characters in the text must be in italic.

There are some technical errors in the text and the references. Please check them. The length, quality and language of the paper are adequate.

Author Response

Dear reviewer

Thank you very much for such kind words about the manuscript, we really appreciate it.

Reviewer 2 Report

General comment

The manuscript ''The Accumulation of Volatile Compounds and the Change in the Morphology of the Leaf Wax Cover Accompanied the "anti-aging" Effect in Anethum graveolens L. Plants Sprayed with 6-BAP'' described the influence of treatment with 6-BAP of two varieties of Anethum graveolens. It was found that tretament with 6-BAP caused change in the division of leaf blades, intensitity of flowering, and in essential oil content. Also, the influence of heat and water togother with 6-BAP was investigated. Article is well done but some things should be added (see comments below).

 Abstract

Line 34: explain abbreviation ''6-BAP''

Line 35: the names of the varieties should be writing on the botanical correct way. Change ''dill plants of the “Uzory” and “Rusich” varieties'' into ''Anethum graveolens L. 'Uzory' and 'Rusich' varieties''

Keywords

Add some other Keywords instead of ''6-BAP'' and ''Anethum graveolens'' which are mentioned in manuscript title

1. Introduction

Line 87: add ''(Anethum graveolens L., family Apiaceae)'' after ''dill'' (because it is mentioned for the first time in main manuscript)

3. Results

Line 132 and 137: cultivars name 'Uzory' and 'Rusich' should be in Normal. Change through all manuscript

Figures 1 and 2: Fig. 1 contain only photographs of cultivar 'Uzory', while fig. 2 contain only photographs of cultivar 'Rusich'. Why authors gave photos of only one cultivar? It will be more useful to see photos of both cultivars.

Line 182: omit space after bracket.

Line 183: add space before ''The''

Add table with all 45 and 46 EO compounds in cultivars 'Uzory' and 'Rusich', respectively.

Please, put Table 1 in Landscape orientation to be more readable.

Line 205: add ''with'' after ''treatment''?

4. Discussion

There is no authors opinion how to explain that treatment with 6-BAP had different effect on leaves morphology, because in one cultivar treatment caused longer while in second shorter segments (lines 132-138). It seems that there is no specific influence of treatment.

Line 263: omit ''s''

Line 298: omit ''(2021)''

Line 300: write scientific plant names with or without authors’ abbreviation (see also lines 247 and 275)

2. Materials and Methods

Line 335: change "Uzory" into 'Uzory'

Line 337: change "Rusich" into 'Rusich'

Lines 374, 375: change letter "x" into symbol "×"

 References

Line 443: ''Dill ('' and ''L.)'' should not to be in Italic

Line 457: the same for ''L.''

Line 491, 492: omit Underline

Lines 495, 496: Umbelliferae

Line 520, 521: omit Underline

Line 563: change letter "x" into symbol "×" before "piperita"

 

Author Response

Dear Reviewer, Thank you for your thoughtful and thorough reading of our manuscript. Our answers to your comments are attached.

Author Response File: Author Response.doc

Round 2

Reviewer 2 Report

My opinion is that the manuscript could be accepted in this form.

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