Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri
Abstract
:1. Introduction
2. Results
2.1. Screening for Antagonistic Endophytic Bacteria
2.2. Strain HF1 Identification
2.3. Analyses of the Antifungal Activity of B. atrophaeus Strain HF1 against V. pyri-Induced Pear Valsa Canker
2.4. The Impact of B. atrophaeus Strain HF1 Culture Filtrate on V. pyri Mycelial Growth and Membrane Permeability
2.5. Amplification of B. atrophaeus Strain HF1 Genes Associated with Lipopeptide Biosynthesis
2.6. The Impact of B. atrophaeus Strain HF1-Derived VOCs on V. pyri Mycelial Growth and Membrane Permeability
2.7. Identification of VOCs Produced by B. atrophaeus Strain HF1
2.8. In Vitro Analyses of the Antifungal Activity of Pure VOCs against V. pyri
2.9. Analyses of the Antifungal Activity of Pure VOCs against Pear Valsa Canker
3. Discussion
4. Materials and Methods
4.1. Fungal Pathogens
4.2. Endophytic Bacterial Isolation and Co-Culture Assays
4.3. Identification of Strain HF1
4.4. In Vivo Analysis of the Antifungal Activity of B. atrophaeus Strain HF1 against V. pyri
4.5. Analyses of the Antifungal Activity of B. atrophaeus Strain HF1 Culture Filtrate against V. pyri Mycelial Growth
4.6. Lipopeptide Biosynthesis-Related Genes Amplification
4.7. Analyses of the Antifungal Activity B. atrophaeus Strain HF1-Derived VOCs
4.8. Effect of B. atrophaeus Strain HF1 Culture Filtrate or VOCs on V. pyri Hyphae Membrane Permeability
4.9. Identification of B. atrophaeus Strain HF1-Derived VOCs
4.10. Analysis of the Antifungal Activity of Individual VOCs against Mycelial Growth of Pathogenic Fungi
4.11. Evaluation of the Antifungal Activity of Specific VOCs against Pear Valsa Canker
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Family | Compound | CAS | Molecular Formula | RT (min) | Area (%) |
---|---|---|---|---|---|
Alcohols | Methanethiol | 74-93-1 | CH4S | 1.87 | 7.69 |
2-pentanol, 3-methyl- | 565-60-6 | C6H14O | 11.87 | 8.49 | |
(±)-5-methyl-2-hexanol | 111768-09-3 | C7H16O | 14.32 | 16.56 | |
2-heptanol, 6-methyl- | 4730-22-7 | C8H18O | 17.01 | 16.67 | |
2-heptanol, 5-methyl- | 54630-50-1 | C8H18O | 17.39 | 14.24 | |
1-hexanol, 4-methyl- | 818-49-5 | C7H16O | 19.15 | 5.50 | |
2-furanmethanol | 98-00-0 | C5H6O2 | 24.10 | 11.15 | |
Octaethylene glycol | 5117-19-1 | C16H34O9 | 40.10 | 4.03 | |
Esters | Methyl thiolacetate | 1534-08-3 | C3H6OS | 6.62 | 19.37 |
Butanoic acid, 2-methyl-, ethyl ester | 7452-79-1 | C7H14O2 | 6.89 | 9.99 | |
Butanoic acid, 3-methyl-, ethyl ester | 108-64-5 | C7H14O2 | 7.38 | 26.80 | |
S-methyl 3-methylbutanethioate | 23747-45-7 | C6H12OS | 11.84 | 8.49 | |
Iberverin | 505-79-3 | C5H9NS2 | 26.35 | 3.84 | |
Methyl methoxyacetate | 6290-49-9 | C4H8O3 | 29.88 | 3.75 | |
Ketones | Bis(4-fluorophenyl)methanone | 345-92-6 | C13H8F2O | 2.04 | 7.21 |
3-methyl-2-butanone | 563-80-4 | C5H10O | 3.72 | 33.41 | |
Methyl Isobutyl Ketone | 108-10-1 | C6H12O | 5.46 | 6.64 | |
4′-methylvalerophenone | 1671-77-8 | C12H16O | 13.48 | 1.28 | |
Hydroxyacetone | 116-09-6 | C3H6O2 | 14.68 | 3.95 | |
Alkanes | Octane, 3-ethyl-2,7-dimethyl- | 62183-55-5 | C12H26 | 14.56 | 3.62 |
Decane, 6-ethyl-2-methyl- | 62108-21-8 | C13H28 | 20.23 | 12.64 | |
Octane, 2,4,6-trimethyl- | 62016-37-9 | C11H24 | 25.03 | 7.01 | |
Ethers | 1-ethoxy-2-propanol | 1569-02-4 | C5H12O2 | 13.09 | 95.76 |
Tri(propylene glycol) methyl ether | 25498-49-1 | C10H22O4 | 41.66 | 5.69 | |
Acids | Guanidinopropionic acid | 353-09-3 | C4H9N3O2 | 4.02 | 39.04 |
Hexanoic acid | 142-62-1 | C6H12O2 | 8.62 | 5.15 | |
Aldehydes | 2-methylvaleraldehyde | 123-15-9 | C6H12O | 12.33 | 25.42 |
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Yuan, H.; Shi, B.; Wang, Z.; Qin, G.; Hou, H.; Tu, H.; Wang, L. Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri. Int. J. Mol. Sci. 2023, 24, 15477. https://doi.org/10.3390/ijms242015477
Yuan H, Shi B, Wang Z, Qin G, Hou H, Tu H, Wang L. Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri. International Journal of Molecular Sciences. 2023; 24(20):15477. https://doi.org/10.3390/ijms242015477
Chicago/Turabian StyleYuan, Hongbo, Bingke Shi, Zhuoni Wang, Genhong Qin, Hui Hou, Hongtao Tu, and Li Wang. 2023. "Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri" International Journal of Molecular Sciences 24, no. 20: 15477. https://doi.org/10.3390/ijms242015477