Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri

Yuan, Hongbo; Shi, Bingke; Wang, Zhuoni; Qin, Genhong; Hou, Hui; Tu, Hongtao; Wang, Li

doi:10.3390/ijms242015477

Open AccessArticle

Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri

by

Hongbo Yuan

^1,2,†,

Bingke Shi

^1,†,

Zhuoni Wang

¹,

Genhong Qin

¹,

Hui Hou

¹,

Hongtao Tu

^1,3 and

Li Wang

^1,*

¹

Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450009, China

²

Western Agricultural Research Center, Chinese Academy of Agricultural Sciences, Changji 831100, China

³

Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang 453004, China

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2023, 24(20), 15477; https://doi.org/10.3390/ijms242015477

Submission received: 14 September 2023 / Revised: 19 October 2023 / Accepted: 20 October 2023 / Published: 23 October 2023

(This article belongs to the Section Molecular Microbiology)

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Abstract

Valsa pyri-induced pear Valsa canker is among the most prevalent diseases to impact pear quality and yields. Biocontrol strategies to control plant disease represent an attractive alternative to the application of fungicides. In this study, the potential utility of Bacillus atrophaeus strain HF1 was assessed as a biocontrol agent against pear Valsa canker. Strain HF1 suppressed V. pyri mycelium growth by 61.20% and induced the development of malformed hyphae. Both culture filtrate and volatile organic compounds (VOCs) derived from strain HF1 were able to antagonize V. pyri growth. Treatment with strain HF1-derived culture filtrate or VOCs also induced the destruction of hyphal cell membranes. Headspace mixtures prepared from strain HF1 were analyzed, leading to the identification of 27 potential VOCs. Of the thirteen pure chemicals tested, iberverin, hexanoic acid, and 2-methylvaleraldehyde exhibited the strongest antifungal effects on V. pyri, with respective EC₅₀ values of 0.30, 6.65, and 74.07 μL L⁻¹. Fumigation treatment of pear twigs with each of these three compounds was also sufficient to prevent the development of pear Valsa canker. As such, these results demonstrate that B. atrophaeus strain HF1 and the volatile compounds iberverin, hexanoic acid, and 2-methylvaleraldehyde exhibit promise as novel candidate biocontrol agents against pear Valsa canker.

Keywords: pear Valsa canker; Valsa pyri; antagonistic biocontrol; Bacillus atrophaeus; volatile organic compounds (VOCs)

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MDPI and ACS Style

Yuan, H.; Shi, B.; Wang, Z.; Qin, G.; Hou, H.; Tu, H.; Wang, L. Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri. Int. J. Mol. Sci. 2023, 24, 15477. https://doi.org/10.3390/ijms242015477

AMA Style

Yuan H, Shi B, Wang Z, Qin G, Hou H, Tu H, Wang L. Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri. International Journal of Molecular Sciences. 2023; 24(20):15477. https://doi.org/10.3390/ijms242015477

Chicago/Turabian Style

Yuan, Hongbo, Bingke Shi, Zhuoni Wang, Genhong Qin, Hui Hou, Hongtao Tu, and Li Wang. 2023. "Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri" International Journal of Molecular Sciences 24, no. 20: 15477. https://doi.org/10.3390/ijms242015477

APA Style

Yuan, H., Shi, B., Wang, Z., Qin, G., Hou, H., Tu, H., & Wang, L. (2023). Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri. International Journal of Molecular Sciences, 24(20), 15477. https://doi.org/10.3390/ijms242015477

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Exploration of the Biocontrol Activity of Bacillus atrophaeus Strain HF1 against Pear Valsa Canker Caused by Valsa pyri

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