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Article

Effects of Florfenicol on Intestinal Histology, Apoptosis and Gut Microbiota of Chinese Mitten Crab (Eriocheir sinensis)

by
Xinping Guo
1,
Ziang Qian
1,
Qiqi Pan
1,
Yuqing Hu
1,
Wangxin Mei
1,
Xiumei Xing
1,
Shaowu Yin
1,2,
Jie Ji
1,2,* and
Kai Zhang
1,2,*
1
College of Marine Science and Engineering, Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, Nanjing Normal University, Nanjing 210023, China
2
Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang 222005, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2023, 24(5), 4412; https://doi.org/10.3390/ijms24054412
Submission received: 12 December 2022 / Revised: 20 February 2023 / Accepted: 21 February 2023 / Published: 23 February 2023
(This article belongs to the Section Molecular Microbiology)
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Abstract

Excessive use of antibiotics in aquaculture causes residues in aquatic animal products and harms human health. However, knowledge of florfenicol (FF) toxicology on gut health and microbiota and their resulting relationships in economic freshwater crustaceans is scarce. Here, we first investigated the influence of FF on the intestinal health of Chinese mitten crabs, and then explored the role of bacterial community in FF-induced intestinal antioxidation system and intestinal homeostasis dysbiosis. A total of 120 male crabs (48.5 ± 4.5 g) were experimentally treated in four different concentrations of FF (0, 0.5, 5 and 50 μg/L) for 14 days. Responses of antioxidant defenses and changes of gut microbiota were assessed in the intestine. Results revealed that FF exposure induced significant histological morphology variation. FF exposure also enhanced immune and apoptosis characteristics in the intestine after 7 days. Moreover, antioxidant enzyme catalase activities showed a similar pattern. The intestinal microbiota community was analyzed based on full-length 16S rRNA sequencing. Only the high concentration group showed a marked decrease in microbial diversity and change in its composition after 14 days of exposure. Relative abundance of beneficial genera increased on day 14. These findings illustrate that exposure to FF could cause intestinal dysfunction and gut microbiota dysbiosis in Chinese mitten crabs, which provides new insights into the relationship between gut health and gut microbiota in invertebrates following exposure to persistent antibiotics pollutants.
Keywords: florfenicol; gut barrier; apoptosis; gut microbiota; crab florfenicol; gut barrier; apoptosis; gut microbiota; crab

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MDPI and ACS Style

Guo, X.; Qian, Z.; Pan, Q.; Hu, Y.; Mei, W.; Xing, X.; Yin, S.; Ji, J.; Zhang, K. Effects of Florfenicol on Intestinal Histology, Apoptosis and Gut Microbiota of Chinese Mitten Crab (Eriocheir sinensis). Int. J. Mol. Sci. 2023, 24, 4412. https://doi.org/10.3390/ijms24054412

AMA Style

Guo X, Qian Z, Pan Q, Hu Y, Mei W, Xing X, Yin S, Ji J, Zhang K. Effects of Florfenicol on Intestinal Histology, Apoptosis and Gut Microbiota of Chinese Mitten Crab (Eriocheir sinensis). International Journal of Molecular Sciences. 2023; 24(5):4412. https://doi.org/10.3390/ijms24054412

Chicago/Turabian Style

Guo, Xinping, Ziang Qian, Qiqi Pan, Yuqing Hu, Wangxin Mei, Xiumei Xing, Shaowu Yin, Jie Ji, and Kai Zhang. 2023. "Effects of Florfenicol on Intestinal Histology, Apoptosis and Gut Microbiota of Chinese Mitten Crab (Eriocheir sinensis)" International Journal of Molecular Sciences 24, no. 5: 4412. https://doi.org/10.3390/ijms24054412

APA Style

Guo, X., Qian, Z., Pan, Q., Hu, Y., Mei, W., Xing, X., Yin, S., Ji, J., & Zhang, K. (2023). Effects of Florfenicol on Intestinal Histology, Apoptosis and Gut Microbiota of Chinese Mitten Crab (Eriocheir sinensis). International Journal of Molecular Sciences, 24(5), 4412. https://doi.org/10.3390/ijms24054412

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