Evidence of the Dysbiotic Effect of Psychotropics on Gut Microbiota and Capacity of Probiotics to Alleviate Related Dysbiosis in a Model of the Human Colon

Round 1

Reviewer 1 Report

The results of this study demonstrate that psychotropic drugs modulate the gut microbiome composition and function, whereas probiotics can minimize dysbiosis related to these drugs.

Numerous researchers have conducted research on this subject in the past. While the manuscript is well written, the data presentation is clear and neat.

The manuscript can be accepted after minor revision.

Section conclusion:
Clearly state the study's significance, potential limitations, and future directions.

Best wishes.

The results of this study demonstrate that psychotropic drugs modulate the gut microbiome composition and function, whereas probiotics can minimize dysbiosis related to these drugs.

Numerous researchers have conducted research on this subject in the past. While the manuscript is well written, the data presentation is clear and neat.

The manuscript can be accepted after minor revision.

Section conclusion:
Clearly state the study's significance, potential limitations, and future directions.

Best wishes.

Author Response

Thank you for your thorough review of our manuscript. We appreciate your positive feedback and your time and effort in providing suggestions for improvement.

We have already addressed your suggestions regarding the significance, potential limitations, and future directions of our study in the manuscript. Specifically, we have discussed these points at the end of the discussion, as highlighted in L289-302 and L303-311.

Reviewer 2 Report

Authors investigated the impact of two psychotropic drugs, aripiprazole and (S)-citalopram, on the gut microbiome composition, as well as the potential of probiotics to attenuate the related dysbiosis using an ex vivo model of the human colon. These findings provide evidence that psychotropics can modulate the composition of the gut microbiome, while probiotics can help to mitigate the related dysbiosis. Overall, the manuscript is clearly written, the methods well designed, and the experimental data generally supports the main conclusions of the paper. My main concern with this manuscript is that Figures 3, 4 and 6 are so small that they are hard to read and interoperate. Could the authors enlarge the elements of these Figures to improve the readability?

Author Response

Thank you for your feedback and helpful suggestions. We have revised the manuscript as per your suggestion and have enlarged the elements of Figures 3, 4, and 6 to improve their readability.