Topical Alginate Protection against Pepsin-Mediated Esophageal Damage: E-Cadherin Proteolysis and Matrix Metalloproteinase Induction
Abstract
:1. Introduction
2. Results
2.1. Cell Viability
2.2. E-Cadherin and ADAM-10 Cleavage
2.3. MMP Expression
2.4. Proliferation Assay
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. ATP Assay
4.3. Western Blot
4.4. Immunofluoresence
4.5. Real Time qPCR
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Samuels, T.L.; Blaine-Sauer, S.; Yan, K.; Plehhova, K.; Coyle, C.; Johnston, N. Topical Alginate Protection against Pepsin-Mediated Esophageal Damage: E-Cadherin Proteolysis and Matrix Metalloproteinase Induction. Int. J. Mol. Sci. 2023, 24, 7932. https://doi.org/10.3390/ijms24097932
Samuels TL, Blaine-Sauer S, Yan K, Plehhova K, Coyle C, Johnston N. Topical Alginate Protection against Pepsin-Mediated Esophageal Damage: E-Cadherin Proteolysis and Matrix Metalloproteinase Induction. International Journal of Molecular Sciences. 2023; 24(9):7932. https://doi.org/10.3390/ijms24097932
Chicago/Turabian StyleSamuels, Tina L., Simon Blaine-Sauer, Ke Yan, Kate Plehhova, Cathal Coyle, and Nikki Johnston. 2023. "Topical Alginate Protection against Pepsin-Mediated Esophageal Damage: E-Cadherin Proteolysis and Matrix Metalloproteinase Induction" International Journal of Molecular Sciences 24, no. 9: 7932. https://doi.org/10.3390/ijms24097932