Triple-Negative Breast Cancer EVs Modulate Growth and Migration of Normal Epithelial Lung Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of EVs from TNBC and MCF10a Cell Lines by NTA and Immunoblotting Analysis
2.2. Cytoxicity Effect of EVs on Normal Human Bronchial Epithelial Cells
2.3. Evaluation of EVs Uptake through Confocal Microscopy
2.4. EVs Promotes Motility of Normal Human Bronchial Epithelial Cells
2.5. BEAS-2B Cells Have Higher Migration Rate in Presence of Breast Cancer-Derived EVs
3. Materials and Methods
3.1. Cell Lines
3.2. EVs Separation from Breast Cancer Cells
3.3. Particle Concentration and Size Using Nanoparticle Tracking Analysis (NTA)
3.4. Immunoblotting Analysis of EVs
3.5. Cytotoxicity Assay (MTS Assay)
3.6. Uptake of EVs Analyzed by Immunofluorescence
3.7. Scratch Assays
3.8. Transwell Migration Assay
3.9. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
BSA | Bovine Serum Albumin |
CL | cell lysate |
CTRL | control |
EMT | epithelial mesenchymal transition |
ER | estrogen receptor |
EVs | extracellular vesicles |
EGFR2 | human epidermal growth factor receptor 2 |
PBS | phosphate saline buffer |
PgR | progesterone receptor |
TEAD | TEA Domain Transcription Factor |
TNBC | Triple-negative breast cancer |
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Leone, I.; Santoro, J.; Soricelli, A.; Febbraro, A.; Santoriello, A.; Carrese, B. Triple-Negative Breast Cancer EVs Modulate Growth and Migration of Normal Epithelial Lung Cells. Int. J. Mol. Sci. 2024, 25, 5864. https://doi.org/10.3390/ijms25115864
Leone I, Santoro J, Soricelli A, Febbraro A, Santoriello A, Carrese B. Triple-Negative Breast Cancer EVs Modulate Growth and Migration of Normal Epithelial Lung Cells. International Journal of Molecular Sciences. 2024; 25(11):5864. https://doi.org/10.3390/ijms25115864
Chicago/Turabian StyleLeone, Ilaria, Jessie Santoro, Andrea Soricelli, Antonio Febbraro, Antonio Santoriello, and Barbara Carrese. 2024. "Triple-Negative Breast Cancer EVs Modulate Growth and Migration of Normal Epithelial Lung Cells" International Journal of Molecular Sciences 25, no. 11: 5864. https://doi.org/10.3390/ijms25115864