Molecular Basis of CO2 Sensing in Hyphantria cunea
Abstract
:1. Introduction
2. Results
2.1. Effect of CO2 on H. cunea Behavior
2.2. Identification and Homology Analysis of HcunGRs
2.3. Electrophysiological Response of the Antennae and Labial Palp to CO2
2.4. Two-Electrode Voltage Clamp (TEVC) Response of HcunGr1, HcunGr2, HcunGr3, and Their Combinations
2.5. Anterograde Dye Filling of Labial Palps in H. cunea
3. Discussion
4. Materials and Methods
4.1. Insect
4.2. Binary Choice Assay
4.3. Homology Analysis of Gustatory Receptors (GRs)
4.4. Electrophysiological Recording
4.5. RNA Extraction, Expression Pattern Analysis, Quantitative PCR, and Cloning
4.6. cRNA Synthesis and Oocyte Microinjection
4.7. CO2 Quantification and TEVC
4.8. Anterograde Dye Filling and Immunohistochemical Staining of the Labial Palps
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, J.; Duan, S.; Wang, W.; Liu, D.; Wang, Y. Molecular Basis of CO2 Sensing in Hyphantria cunea. Int. J. Mol. Sci. 2024, 25, 5987. https://doi.org/10.3390/ijms25115987
Zhang J, Duan S, Wang W, Liu D, Wang Y. Molecular Basis of CO2 Sensing in Hyphantria cunea. International Journal of Molecular Sciences. 2024; 25(11):5987. https://doi.org/10.3390/ijms25115987
Chicago/Turabian StyleZhang, Jian, Shiwen Duan, Wenlong Wang, Duo Liu, and Yinliang Wang. 2024. "Molecular Basis of CO2 Sensing in Hyphantria cunea" International Journal of Molecular Sciences 25, no. 11: 5987. https://doi.org/10.3390/ijms25115987