PvdL Orchestrates the Assembly of the Nonribosomal Peptide Synthetases Involved in Pyoverdine Biosynthesis in Pseudomonas aeruginosa
Abstract
:1. Introduction
2. Results
2.1. Localizations of NRPSs in Cells
2.2. Diffusion of NRPSs in Living Cells
2.3. NRPS-NRPS Interactions
2.4. Co-Localizations of NRPSs
3. Discussion
4. Materials and Methods
4.1. Bacteria Mutants, Cell Culture, and Labeling
4.2. Microscopy and Analysis
4.3. FLIM-FRET
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
NRPS | Nonribosomal peptide synthetase |
FLIM-FRET | Förster resonance energy transfer measured by fluorescence lifetime imaging |
IQR | Interquartile range |
MSD | Mean square displacement |
JD | Jump distance |
OD | Optical density |
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Strain Name | (ns) | (ns) | FRET Efficiency |
---|---|---|---|
eGFP-PvdL/PvdJ-mCherry | 2.3 | 2.3 | 0.00 |
PvdJ-eGFP/mCherry-PvdL | 2.3 | 2.3 | 0.00 |
eGFP-PvdL/mCherry-PvdD | 2.3 | 2.0 | 0.13 |
eGFP-PvdL/PvdI-mCherry | 2.3 | 2.2 | 0.04 |
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Manko, H.; Steffan, T.; Gasser, V.; Mély, Y.; Schalk, I.; Godet, J. PvdL Orchestrates the Assembly of the Nonribosomal Peptide Synthetases Involved in Pyoverdine Biosynthesis in Pseudomonas aeruginosa. Int. J. Mol. Sci. 2024, 25, 6013. https://doi.org/10.3390/ijms25116013
Manko H, Steffan T, Gasser V, Mély Y, Schalk I, Godet J. PvdL Orchestrates the Assembly of the Nonribosomal Peptide Synthetases Involved in Pyoverdine Biosynthesis in Pseudomonas aeruginosa. International Journal of Molecular Sciences. 2024; 25(11):6013. https://doi.org/10.3390/ijms25116013
Chicago/Turabian StyleManko, Hanna, Tania Steffan, Véronique Gasser, Yves Mély, Isabelle Schalk, and Julien Godet. 2024. "PvdL Orchestrates the Assembly of the Nonribosomal Peptide Synthetases Involved in Pyoverdine Biosynthesis in Pseudomonas aeruginosa" International Journal of Molecular Sciences 25, no. 11: 6013. https://doi.org/10.3390/ijms25116013