Analysis of the BDNF Gene rs6265 Polymorphism in a Group of Women with Alcohol Use Disorder, Taking into Account Personality Traits
Abstract
:1. Introduction
2. Results
2.1. STAI Trait Scale
2.2. STAI State Scale
2.3. Neuroticism Scale
2.4. Agreeability Scale
3. Discussion
4. Materials and Methods
4.1. Participants
4.2. Psychometric Tests
4.3. Genotyping
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hardy-Weinberg Equilibrium, Including Analysis for Ascertainment Bias | Observed (Expected) | Allele Freq | χ2 (p-Value) | |
---|---|---|---|---|
BDNF rs6265 | ||||
AUD subjects n = 101 | G/A | 19 (21.9) | p (A)= 0.88 q (G)= 0.12 | 1.777 (0.1824) |
G/G | 79 (77.5) | |||
A/A | 3 (1.5) | |||
Control subjects n = 112 | G/A | 42 (36.6) | p (A)= 0.79 q (G)= 0.21 | 2.486 (0.1148) |
G/G | 68 (70.7) | |||
A/A | 2 (4.7) |
BDNF rs6265 | |||||
---|---|---|---|---|---|
Genotypes | Alleles | ||||
G/A n (%) | G/G n (%) | A/A n (%) | G n (%) | A n (%) | |
AUD n = 101 | 19 (18.81%) | 79 (78.22%) | 3 (2.97%) | 177 (87.62%) | 25 (12.38%) |
Control n = 112 | 42 (37.50%) | 68 (60.71%) | 2 (1.79%) | 178 (79.46%) | 52 (20.54%) |
χ2 (p-value) | 9.152 (0.0103 *) | 5.091 (0.0240 *) |
State-Trait Anxiety Inventory NEO Five-Factor Inventory | AUD (n = 101) | Control (n = 112) | Z | (p-Value) |
---|---|---|---|---|
Anxiety trait | 7.49 ± 2.20 | 4.90 ± 2.30 | 7.284 | 0.0000 * |
Anxiety state | 6.02 ± 2.51 | 4.55 ± 2.15 | 4.417 | 0.0000 * |
Neuroticism | 7.26 ± 1.86 | 4.55 ± 2.09 | 8.287 | 0.0000 * |
Extraversion | 5.11 ± 2.24 | 6.72 ± 1.91 | −5.051 | 0.0000 * |
Openness | 5.11 ± 2.14 | 4.50 ± 1.66 | 2.472 | 0.0134 * |
Agreeability | 3.84 ± 1.87 | 5.49 ± 2.19 | −5.380 | 0.0000 * |
Conscientiousness | 4.97 ± 2.33 | 6.88 ± 2.00 | −5.844 | 0.0000 * |
STAI NEO FFI | Group | BDNF rs6265 | ANOVA | |||||
---|---|---|---|---|---|---|---|---|
G/A n = 61 M ± SD | G/G n = 147 M ± SD | A/A n = 5 M ± SD | Factor | F (p-Value) | ɳ2 | Power (Alpha = 0.05) | ||
STAI trait scale | Alcohol use disorder (AUD); n = 101 | 8.00 ± 1.45 | 7.33 ± 2.35 | 8.67 ± 1.15 | AUD/control BDNF rs6265 AUD/control x BDNF rs6265 | F1,207 = 14.42 (p = 0.0001) * F2,207 = 1.38 (p = 0.2541) F2,207 = 4.33 (p = 0.0143) * | 0.065 0.013 0.040 | 0.966 0.294 0.747 |
Control; n = 112 | 4.00 ± 2.24 | 5.41 ± 2.19 | 6.50 ± 0.71 | |||||
STAI state scale | Alcohol use disorder (AUD); n = 101 | 7.00 ± 1.97 | 5.73 ± 2.59 | 7.33 ± 1.15 | AUD/control BDNF rs6265 AUD/control x BDNF rs6265 | F1,207 = 6.07 (p < 0.0145) * F2,207 = 0.81 (p = 0.4447) F2,207 = 7.32 (p = 0.00085) * | 0.029 0.008 0.066 | 0.689 0.188 0.935 |
Control; n = 112 | 3.59 ± 1.96 | 5.10 ± 2.09 | 6.00 ± 0.71 | |||||
Neuroticism scale | Alcohol use disorder (AUD); n = 101 | 7.47 ± 1.39 | 7.25 ± 1.96 | 6.00 ± 1.73 | AUD/control BDNF rs6265 AUD/control x BDNF rs6265 | F1,207 = 11.60 (p < 0.0008) * F2,207 = 1.19 (p = 0.3058) F2,207 = 3.17 (p = 0.0440) * | 0.053 0.011 0.030 | 0.924 0.259 0.603 |
Control; n = 112 | 3.81 ± 1.80 | 4.99 ± 2.17 | 5.50 ± 0.71 | |||||
Extraversion scale | Alcohol use disorder (AUD); n = 101 | 5.53 ± 2.34 | 4.94 ± 2.16 | 7.00 ± 3.46 | AUD/control BDNF rs6265 AUD/control x BDNF rs6265 | F1,207 = 0.54 (p = 0.4618) * F2,207 = 0.22 (p = 0.8013) F2,207 = 2.08 (p = 0.1272) | 0.003 0.002 0.020 | 0.114 0.084 0.425 |
Control; n = 112 | 6.62 ± 1.74 | 6.82 ± 2.02 | 5.50 ± 2.12 | |||||
Openness scale | Alcohol use disorder (AUD); n = 101 | 4.79 ± 1.81 | 5.25 ± 2.20 | 3.33 ± 2.08 | AUD/control BDNF rs6265 AUD/control x BDNF rs6265 | F1,207 = 0.003 (p = 0.9503) F2,207 = 0.86 (p = 0.4248) F2,207 = 0.76 (p = 0.4692) | 0.00001 0.008 0.007 | 0.050 0.197 0.178 |
Control; n = 112 | 4.48 ± 1.58 | 4.51 ± 1.74 | 4.50 ± 0.71 | |||||
Agreeability scale | Alcohol use disorder (AUD); n = 101 | 4.84 ± 1.42 | 3.67 ± 1.89 | 2.00 ± 1.00 | AUD/control BDNF rs6265 AUD/control x BDNF rs6265 | F1,207 = 10.70 (p = 0.0013) F2,207 = 1.42 (p = 0.2432) F2,207 = 3.10 (p = 0.0473) * | 0.049 0.014 0.029 | 0.902 0.303 0.592 |
Control; n = 112 | 5.36 ± 2.33 | 5.56 ± 2.13 | 6.00 ± 1.41 | |||||
Conscientiousness scale | Alcohol use disorder (AUD); n = 101 | 5.16 ± 2.17 | 4.92 ± 2.41 | 5.00 ± 1.73 | AUD/control BDNF rs6265 AUD/control x BDNF rs6265 | F1,207 = 3.44 (p = 0.0649) F2,207 = 1.12 (p = 0.3276) F2,207 = 0.57 (p = 0.5690) | 0.016 0.011 0.005 | 0.455 0.246 0.143 |
Control; n = 112 | 7.29 ± 1.77 | 6.69 ± 2.11 | 5.00 ± 1.41 |
BDNF rs6265 and Anxiety Trait Scale | ||||||
{1} M = 8.00 | {2} M = 7.33 | {3} M = 8.67 | {4} M = 4.00 | {5} M = 5.41 | {6} M = 6.50 | |
AUD subjects G/A {1} | 0.2339 | 0.6261 | 0.0000 * | 0.0000 * | 0.3599 | |
AUD subjects G/G {2} | 0.3023 | 0.0000 * | 0.0000 * | 0.5991 | ||
AUD subjects A/A {3} | 0.0005 * | 0.0129 * | 0.2817 | |||
Control G/A {4} | 0.0013 * | 0.1178 | ||||
Control G/G {5} | 0.4911 | |||||
Control A/A {6} | ||||||
BDNF rs6265 and Anxiety State Scale | ||||||
{1} M = 7.00 | {2} M = 5.73 | {3} M = 7.33 | {4} M = 3.60 | {5} M = 5.10 | {6} M = 6.00 | |
AUD subjects G/A {1} | 0.0288 * | 0.8118 | 0.0000 * | 0.0014 * | 0.5507 | |
AUD subjects G/G {2} | 0.2284 | 0.0000 * | 0.0915 | 0.8691 | ||
AUD subjects A/A {3} | 0.0059 * | 0.0945 | 0.5171 | |||
Control G/A {4} | 0.0008 * | 0.1414 | ||||
Control G/G {5} | 0.5791 | |||||
Control A/A {6} |
BDNF rs6265 and Neuroticism Scale | ||||||
{1} M = 7.47 | {2} M = 7.25 | {3} M = 6.00 | {4} M = 3.81 | {5} M = 4.99 | {6} M = 5.50 | |
AUD subjects G/A {1} | 0.6586 | 0.2253 | 0.0000 * | 0.0000 * | 0.1750 | |
AUD subjects G/G {2} | 0.2760 | 0.0000 * | 0.0000 * | 0.2108 | ||
AUD subjects A/A {3} | 0.0616 | 0.3789 | 0.7792 | |||
Control G/A {4} | 0.0024 * | 0.2325 | ||||
Control G/G {5} | 0.7134 | |||||
Control A/A {6} | ||||||
BDNF rs6265 and Agreeability Scale | ||||||
{1} M = 4.84 | {2} M = 3.67 | {3} M = 2.00 | {4} M = 5.36 | {5} M = 5.56 | {6} M = 6.00 | |
AUD subjects G/A {1} | 0.0244 * | 0.0247 * | 0.3580 | 0.1734 | 0.4420 | |
AUD subjects G/G {2} | 0.1616 | 0.0000 * | 0.0000 * | 0.1092 | ||
AUD subjects A/A {3} | 0.0060 * | 0.0032 * | 0.0314 * | |||
Control G/A {4} | 0.6118 | 0.6609 | ||||
Control G/G {5} | 0.7613 | |||||
Control A/A {6} |
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Boroń, A.; Suchanecka, A.; Chmielowiec, K.; Chmielowiec, J.; Masiak, J.; Trybek, G.; Strońska-Pluta, A.; Rychel, M.; Grzywacz, A. Analysis of the BDNF Gene rs6265 Polymorphism in a Group of Women with Alcohol Use Disorder, Taking into Account Personality Traits. Int. J. Mol. Sci. 2024, 25, 6448. https://doi.org/10.3390/ijms25126448
Boroń A, Suchanecka A, Chmielowiec K, Chmielowiec J, Masiak J, Trybek G, Strońska-Pluta A, Rychel M, Grzywacz A. Analysis of the BDNF Gene rs6265 Polymorphism in a Group of Women with Alcohol Use Disorder, Taking into Account Personality Traits. International Journal of Molecular Sciences. 2024; 25(12):6448. https://doi.org/10.3390/ijms25126448
Chicago/Turabian StyleBoroń, Agnieszka, Aleksandra Suchanecka, Krzysztof Chmielowiec, Jolanta Chmielowiec, Jolanta Masiak, Grzegorz Trybek, Aleksandra Strońska-Pluta, Monika Rychel, and Anna Grzywacz. 2024. "Analysis of the BDNF Gene rs6265 Polymorphism in a Group of Women with Alcohol Use Disorder, Taking into Account Personality Traits" International Journal of Molecular Sciences 25, no. 12: 6448. https://doi.org/10.3390/ijms25126448