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Article

The Expression of Cell Cycle Cyclins in a Human Megakaryoblast Cell Line Exposed to Simulated Microgravity

by
Alisa A. Sokolovskaya
*,
Ekaterina A. Sergeeva
,
Arkadiy A. Metelkin
,
Mikhail A. Popov
,
Irina A. Zakharova
and
Sergey G. Morozov
Department of Molecular and Cellular Pathophysiology, Institute of General Pathology and Pathophysiology, Baltiyskaya Str. 8, 125315 Moscow, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(12), 6484; https://doi.org/10.3390/ijms25126484
Submission received: 30 April 2024 / Revised: 24 May 2024 / Accepted: 6 June 2024 / Published: 12 June 2024

Abstract

The study of the physiological and pathophysiological processes under extreme conditions facilitates a better understanding of the state of a healthy organism and can also shed light on the pathogenesis of diseases. In recent years, it has become evident that gravitational stress affects both the whole organism and individual cells. We have previously demonstrated that simulated microgravity inhibits proliferation, induces apoptosis, changes morphology, and alters the surface marker expression of megakaryoblast cell line MEG-01. In the present work, we investigate the expression of cell cycle cyclins in MEG-01 cells. We performed several experiments for 24 h, 72 h, 96 h and 168 h. Flow cytometry and Western blot analysis demonstrated that the main change in the levels of cyclins expression occurs under conditions of simulated microgravity after 96 h. Thus, the level of cyclin A expression showed an increase in the RPM group during the first 4 days, followed by a decrease, which, together with the peak of cyclin D, may indicate inhibition of the cell cycle in the G2 phase, before mitosis. In addition, based on the data obtained by PCR analysis, we were also able to see that both cyclin A and cyclin B expression showed a peak at 72 h, followed by a gradual decrease at 96 h. STED microscopy data also confirmed that the main change in cyclin expression of MEG-01 cells occurs at 96 h, under simulated microgravity conditions, compared to static control. These results suggested that the cell cycle disruption induced by RPM-simulated microgravity in MEG-01 cells may be associated with the altered expression of the main regulators of the cell cycle. Thus, these data implicate the development of cellular stress in MEG-01 cells, which may be important for proliferating human cells exposed to microgravity in real space.
Keywords: microgravity; random positioning machine; megakaryocytes; cell cycle; cyclins microgravity; random positioning machine; megakaryocytes; cell cycle; cyclins

Share and Cite

MDPI and ACS Style

Sokolovskaya, A.A.; Sergeeva, E.A.; Metelkin, A.A.; Popov, M.A.; Zakharova, I.A.; Morozov, S.G. The Expression of Cell Cycle Cyclins in a Human Megakaryoblast Cell Line Exposed to Simulated Microgravity. Int. J. Mol. Sci. 2024, 25, 6484. https://doi.org/10.3390/ijms25126484

AMA Style

Sokolovskaya AA, Sergeeva EA, Metelkin AA, Popov MA, Zakharova IA, Morozov SG. The Expression of Cell Cycle Cyclins in a Human Megakaryoblast Cell Line Exposed to Simulated Microgravity. International Journal of Molecular Sciences. 2024; 25(12):6484. https://doi.org/10.3390/ijms25126484

Chicago/Turabian Style

Sokolovskaya, Alisa A., Ekaterina A. Sergeeva, Arkadiy A. Metelkin, Mikhail A. Popov, Irina A. Zakharova, and Sergey G. Morozov. 2024. "The Expression of Cell Cycle Cyclins in a Human Megakaryoblast Cell Line Exposed to Simulated Microgravity" International Journal of Molecular Sciences 25, no. 12: 6484. https://doi.org/10.3390/ijms25126484

APA Style

Sokolovskaya, A. A., Sergeeva, E. A., Metelkin, A. A., Popov, M. A., Zakharova, I. A., & Morozov, S. G. (2024). The Expression of Cell Cycle Cyclins in a Human Megakaryoblast Cell Line Exposed to Simulated Microgravity. International Journal of Molecular Sciences, 25(12), 6484. https://doi.org/10.3390/ijms25126484

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