Association between Circulating T Cells and the Gut Microbiome in Healthy Individuals: Findings from a Pilot Study
Abstract
:1. Introduction
2. Results
2.1. Cross-Sectional Association between the Percentage of T Cell Subsets and Relative Abundance of Bacterial Genera
2.2. Longitudinal Analysis Percentage of T Cell Subsets at the Baseline and Fold Change of Bacterial Genera over 6 Weeks
3. Discussion
Strengths and Limitations
4. Material and Methods
4.1. Study Design
4.2. Collection and Processing of the Stool Samples
4.3. Data Preprocessing
4.4. Collection of Blood Samples and Measurement of T Cell Subsets
4.5. Covariates
4.6. Statistical Analysis
4.7. Sparse Canonical Correlation Analysis (sCCA)
4.8. Linear Regression Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Immune Cell | Estimate | Standard Error | p-Value | Bacterial Genera (Phylum) |
---|---|---|---|---|
Naïve CD4+ | 0.03 | 0.01 | <0.01 | Intestinimonas (Bacillota) |
Activated CD8+ | −0.11 | 0.05 | 0.03 | Cellulosibacter (Bacillota) |
Immune Cell | Estimate | Standard Error | p-Value | Bacterial Genera (Phylum) |
---|---|---|---|---|
Activated CD4+ | −0.07 | 0.03 | 0.03 | Anaerovorax (Bacillota) |
Terminal Effector CD4+ | 0.05 | 0.02 | 0.01 | Flavonifractor (Bacillota) |
Naïve CD4+ | −0.03 | 0.03 | 0.02 | Clostridium_XlVb (Bacillota) |
Microbiome | Immune Cells and Time Point | Estimate (p-Value) | |
---|---|---|---|
Intestinimonas | Naïve CD4+ | Baseline | 0.03 (<0.01) |
Clostridium_XlVb | 6-weeks change | −0.03 (0.02) |
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Vivek, S.; Shen, Y.S.; Guan, W.; Onyeaghala, G.; Oyenuga, M.; Staley, C.; Karger, A.B.; Prizment, A.E.; Thyagarajan, B. Association between Circulating T Cells and the Gut Microbiome in Healthy Individuals: Findings from a Pilot Study. Int. J. Mol. Sci. 2024, 25, 6831. https://doi.org/10.3390/ijms25136831
Vivek S, Shen YS, Guan W, Onyeaghala G, Oyenuga M, Staley C, Karger AB, Prizment AE, Thyagarajan B. Association between Circulating T Cells and the Gut Microbiome in Healthy Individuals: Findings from a Pilot Study. International Journal of Molecular Sciences. 2024; 25(13):6831. https://doi.org/10.3390/ijms25136831
Chicago/Turabian StyleVivek, Sithara, You Shan Shen, Weihua Guan, Guillaume Onyeaghala, Mosunmoluwa Oyenuga, Christopher Staley, Amy B. Karger, Anna E. Prizment, and Bharat Thyagarajan. 2024. "Association between Circulating T Cells and the Gut Microbiome in Healthy Individuals: Findings from a Pilot Study" International Journal of Molecular Sciences 25, no. 13: 6831. https://doi.org/10.3390/ijms25136831