Effects of Chronic Exposure to Low Doses of Rotenone on Dopaminergic and Cholinergic Neurons in the CNS of Hemigrapsus sanguineus
Abstract
:1. Introduction
2. Results
2.1. Effects of Rotenone Exposure on Motor Behavior and Survival
2.1.1. Open Field Test
2.1.2. Survival Rate
2.2. Effect of Chronic Exposure to Low Doses of Rotenone on Dopaminergic and Cholinergic Immunoreactivity in the CNS of Hemigrapsus sanguineus
2.2.1. TH-lir after Chronic Exposure to Low Doses of Rotenone
2.2.2. ChAT-lir after Chronic Exposure to Low Doses of Rotenone
3. Discussion
4. Materials and Methods
4.1. Experimental Animals
4.2. Behavioral Assessment
Open Field Test
4.3. Antibodies
4.4. Immunocytochemistry
4.5. Microscopy and Imaging
4.6. Quantification and Statistical Analysis
4.7. Neuroanatomical Nomenclature
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | N | Observed | Expected | (0 − E)2/E | (0 − E)2/V |
---|---|---|---|---|---|
Control | 23 | 1 | 1.950 | 0.463 | 0.798 |
Intact | 24 | 0 | 2.199 | 2.199 | 4.130 |
Rotenone | 11 | 4 | 0.851 | 11.655 | 14.744 |
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Kotsyuba, E.; Dyachuk, V. Effects of Chronic Exposure to Low Doses of Rotenone on Dopaminergic and Cholinergic Neurons in the CNS of Hemigrapsus sanguineus. Int. J. Mol. Sci. 2024, 25, 7159. https://doi.org/10.3390/ijms25137159
Kotsyuba E, Dyachuk V. Effects of Chronic Exposure to Low Doses of Rotenone on Dopaminergic and Cholinergic Neurons in the CNS of Hemigrapsus sanguineus. International Journal of Molecular Sciences. 2024; 25(13):7159. https://doi.org/10.3390/ijms25137159
Chicago/Turabian StyleKotsyuba, Elena, and Vyacheslav Dyachuk. 2024. "Effects of Chronic Exposure to Low Doses of Rotenone on Dopaminergic and Cholinergic Neurons in the CNS of Hemigrapsus sanguineus" International Journal of Molecular Sciences 25, no. 13: 7159. https://doi.org/10.3390/ijms25137159