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Recent Research on Cell and Molecular Biology
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Recent Research on Cell and Molecular Biology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: 10 June 2024 | Viewed by 4263

Special Issue Editor

Dr. Luis Felipe Jiménez-García

E-Mail Website
Guest Editor
Facultad de Ciencias, Universidad Nacional Autónoma de México, Mexico City, Mexico
Interests: microbes; ultrastructure; cell biology

Special Issue Information

Dear Colleagues,

The “Cell and Molecular Biology” Special Issue in IJMS welcomes original research, reviews, and short communication papers covering a broad range of topics that include:

  • Emerging single-cell analysis technologies;
  • Epigenetic modifications;
  • Non-coding RNA molecules;
  • Cellular metabolism in disease;
  • Novel therapeutic targets;
  • CRISPR gene editing;
  • Stem cells in regenerative medicine;
  • Microbiome influence;
  • Cancer biology;
  • Immunology;
  • Cell structure and ultrastructure.

Various interdisciplinary approaches such as genomics, proteomics, bioinformatics, and functional genomics are also highlighted. The aim of this Special Issue is to provide an opportunity for researchers to exchange ideas, promote collaboration, and share the latest scientific discoveries in this dynamic and exciting field of Cell and Molecular Biology. Innovative methods and cutting-edge technologies are welcomed, and the published works will contribute to the scientific community, leading to future progress and advancements in the field.

Dr. Luis Felipe Jiménez-García
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  1. Gene expression
  2. Cellular signaling
  3. Computational biology
  4. Molecular mechanisms
  5. Signal transduction
  6. Epigenetics
  7. Proteomics
  8. Transcriptomics
  9. DNA repair
  10. Stem cells
  11. Apoptosis
  12. Cancer biology
  13. Synthetic biology
  14. Drug discovery
  15. Molecular imaging
  16. Genome engineering
  17. CRISPR/Cas9
  18. Immunology
  19. Neurobiology
  20. Metabolism
  21. RNA Biology

Published Papers (3 papers)

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Research

19 pages, 20639 KiB  
Article
Microscopic Analysis of Nuclear Speckles in a Viviparous Reptile
by Jeniffer Acosta-Cárdenas, Luis Felipe Jiménez-García, Sarai de Jesús Cruz-Gómez, Ana Paulina Mendoza-von der Borch and María de Lourdes Segura-Valdez
Int. J. Mol. Sci. 2024, 25(10), 5281; https://doi.org/10.3390/ijms25105281 (registering DOI) - 12 May 2024
Viewed by 364
Abstract
Nuclear speckles are compartments enriched in splicing factors present in the nucleoplasm of eucaryote cells. Speckles have been studied in mammalian culture and tissue cells, as well as in some non-mammalian vertebrate cells and invertebrate oocytes. In mammals, their morphology is linked to [...] Read more.
Nuclear speckles are compartments enriched in splicing factors present in the nucleoplasm of eucaryote cells. Speckles have been studied in mammalian culture and tissue cells, as well as in some non-mammalian vertebrate cells and invertebrate oocytes. In mammals, their morphology is linked to the transcriptional and splicing activities of the cell through a recruitment mechanism. In rats, speckle morphology depends on the hormonal cycle. In the present work, we explore whether a similar situation is also present in non-mammalian cells during the reproductive cycle. We studied the speckled pattern in several tissues of a viviparous reptile, the lizard Sceloporus torquatus, during two different stages of reproduction. We used immunofluorescence staining against splicing factors in hepatocytes and oviduct epithelium cells and fluorescence and confocal microscopy, as well as ultrastructural immunolocalization and EDTA contrast in Transmission Electron Microscopy. The distribution of splicing factors in the nucleoplasm of oviductal cells and hepatocytes coincides with the nuclear-speckled pattern described in mammals. Ultrastructurally, those cell types display Interchromatin Granule Clusters and Perichromatin Fibers. In addition, the morphology of speckles varies in oviduct cells at the two stages of the reproductive cycle analyzed, paralleling the phenomenon observed in the rat. The results show that the morphology of speckles in reptile cells depends upon the reproductive stage as it occurs in mammals. Full article
(This article belongs to the Special Issue Recent Research on Cell and Molecular Biology)
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30 pages, 7308 KiB  
Article
Effects of S-Adenosylhomocysteine Hydrolase Downregulation on Wnt Signaling Pathway in SW480 Cells
by Ivana Pavičić, Filip Rokić and Oliver Vugrek
Int. J. Mol. Sci. 2023, 24(22), 16102; https://doi.org/10.3390/ijms242216102 - 8 Nov 2023
Cited by 1 | Viewed by 1430
Abstract
S-adenosylhomocysteine hydrolase (AHCY) deficiency results mainly in hypermethioninemia, developmental delay, and is potentially fatal. In order to shed new light on molecular aspects of AHCY deficiency, in particular any changes at transcriptome level, we enabled knockdown of AHCY expression in the colon cancer [...] Read more.
S-adenosylhomocysteine hydrolase (AHCY) deficiency results mainly in hypermethioninemia, developmental delay, and is potentially fatal. In order to shed new light on molecular aspects of AHCY deficiency, in particular any changes at transcriptome level, we enabled knockdown of AHCY expression in the colon cancer cell line SW480 to simulate the environment occurring in AHCY deficient individuals. The SW480 cell line is well known for elevated AHCY expression, and thereby represents a suitable model system, in particular as AHCY expression is regulated by MYC, which, on the other hand, is involved in Wnt signaling and the regulation of Wnt-related genes, such as the β-catenin co-transcription factor LEF1 (lymphoid enhancer-binding factor 1). We selected LEF1 as a potential target to investigate its association with S-adenosylhomocysteine hydrolase deficiency. This decision was prompted by our analysis of RNA-Seq data, which revealed significant changes in the expression of genes related to the Wnt signaling pathway and genes involved in processes responsible for epithelial-mesenchymal transition (EMT) and cell proliferation. Notably, LEF1 emerged as a common factor in these processes, showing increased expression both on mRNA and protein levels. Additionally, we show alterations in interconnected signaling pathways linked to LEF1, causing gene expression changes with broad effects on cell cycle regulation, tumor microenvironment, and implications to cell invasion and metastasis. In summary, we provide a new link between AHCY deficiency and LEF1 serving as a mediator of changes to the Wnt signaling pathway, thereby indicating potential connections of AHCY expression and cancer cell phenotype, as Wnt signaling is frequently associated with cancer development, including colorectal cancer (CRC). Full article
(This article belongs to the Special Issue Recent Research on Cell and Molecular Biology)
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14 pages, 4313 KiB  
Article
Tyrosine Kinase Inhibitor Profiling Using Multiple Forskolin-Responsive Reporter Cells
by Yamato Kasahara, Sakura Tamamura, Gen Hiyama, Motoki Takagi, Kazuya Nakamichi, Yuta Doi, Kentaro Semba, Shinya Watanabe and Kosuke Ishikawa
Int. J. Mol. Sci. 2023, 24(18), 13863; https://doi.org/10.3390/ijms241813863 - 8 Sep 2023
Viewed by 1361
Abstract
We have developed a highly sensitive promoter trap vector system using transposons to generate reporter cells with high efficiency. Using an EGFP/luciferase reporter cell clone responsive to forskolin, which is thought to activate adenylate cyclase, isolated from human chronic myelogenous leukemia cell line [...] Read more.
We have developed a highly sensitive promoter trap vector system using transposons to generate reporter cells with high efficiency. Using an EGFP/luciferase reporter cell clone responsive to forskolin, which is thought to activate adenylate cyclase, isolated from human chronic myelogenous leukemia cell line K562, we found several compounds unexpectedly caused reporter responses. These included tyrosine kinase inhibitors such as dasatinib and cerdulatinib, which were seemingly unrelated to the forskolin-reactive pathway. To investigate whether any other clones of forskolin-responsive cells would show the same response, nine additional forskolin-responsive clones, each with a unique integration site, were generated and quantitatively evaluated by luciferase assay. The results showed that each clone represented different response patterns to the reactive compounds. Also, it became clear that each of the reactive compounds could be profiled as a unique pattern by the 10 reporter clones. When other TKIs, mainly bcr-abl inhibitors, were evaluated using a more focused set of five reporter clones, they also showed unique profiling. Among them, dasatinib and bosutinib, and imatinib and bafetinib showed homologous profiling. The tyrosine kinase inhibitors mentioned above are approved as anticancer agents, and the system could be used for similarity evaluation, efficacy prediction, etc., in the development of new anticancer agents. Full article
(This article belongs to the Special Issue Recent Research on Cell and Molecular Biology)
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

1. Identification LCK as master regulator of TNBC neutrophils enrichment and polarization: in silico reanalysis with extensive systems biology approach

2. TNBC cells effect on neutrophils survival, migration and polarization. An In vitro study using cell lines and healthy neutrophils cocultured with TNBC Vs Luminal Cell lines

3. Tyrosine Kinase Inhibitor Profiling Using Multiple XXXX-responsive Reporter Cells"

4. Identification of XXXX upregulated by stressors that stimulate ATF4"

5. Microscopic  analysis of nuclear speckles in the vivíparous lizard Sceloporus torquatus-Guest Editor

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