Next Article in Journal
Size Matters: Rethinking Hertz Model Interpretation for Cell Mechanics Using AFM
Previous Article in Journal
MyD88 Signaling Accompanied by Microbiota Changes Supports Urinary Bladder Carcinogenesis
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Transcriptome Analysis Reveals the Role of Sucrose in the Production of Latilactobacillus sakei L3 Exopolysaccharide

1
School of Life Sciences, Shanxi Normal University, Taiyuan 030000, China
2
School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(13), 7185; https://doi.org/10.3390/ijms25137185
Submission received: 2 June 2024 / Revised: 21 June 2024 / Accepted: 25 June 2024 / Published: 29 June 2024
(This article belongs to the Section Molecular Microbiology)

Abstract

Latilactobacillus (L.) sakei is a species of lactic acid bacteria (LAB) mostly studied according to its application in food fermentation. Previously, L. sakei L3 was isolated by our laboratory and possessed the capability of high exopolysaccharide (EPS) yield during sucrose-added fermentation. However, the understanding of sucrose promoting EPS production is still limited. Here, we analyzed the growth characteristics of L. sakei L3 and alterations of its transcriptional profiles during sucrose-added fermentation. The results showed that L. sakei L3 could survive between pH 4.0 and pH 9.0, tolerant to NaCl (<10%, w/v) and urea (<6%, w/v). Meanwhile, transcriptomic analysis showed that a total of 426 differentially expressed genes and eight non-coding RNAs were identified. Genes associated with sucrose metabolism were significantly induced, so L. sakei L3 increased the utilization of sucrose to produce EPS, while genes related to uridine monophosphate (UMP), fatty acids and folate synthetic pathways were significantly inhibited, indicating that L. sakei L3 decreased self-growth, substance and energy metabolism to satisfy EPS production. Overall, transcriptome analysis provided valuable insights into the mechanisms by which L. sakei L3 utilizes sucrose for EPS biosynthesis. The study provided a theoretical foundation for the further application of functional EPS in the food industry.
Keywords: Latilactobacillus sakei; synthetic pathway; molecular docking; non-coding RNA; differentially expressed genes Latilactobacillus sakei; synthetic pathway; molecular docking; non-coding RNA; differentially expressed genes

Share and Cite

MDPI and ACS Style

Wang, B.; Wu, B.; Xu, M.; Zuo, K.; Han, Y.; Zhou, Z. Transcriptome Analysis Reveals the Role of Sucrose in the Production of Latilactobacillus sakei L3 Exopolysaccharide. Int. J. Mol. Sci. 2024, 25, 7185. https://doi.org/10.3390/ijms25137185

AMA Style

Wang B, Wu B, Xu M, Zuo K, Han Y, Zhou Z. Transcriptome Analysis Reveals the Role of Sucrose in the Production of Latilactobacillus sakei L3 Exopolysaccharide. International Journal of Molecular Sciences. 2024; 25(13):7185. https://doi.org/10.3390/ijms25137185

Chicago/Turabian Style

Wang, Binbin, Baomei Wu, Min Xu, Kaiyue Zuo, Ye Han, and Zhijiang Zhou. 2024. "Transcriptome Analysis Reveals the Role of Sucrose in the Production of Latilactobacillus sakei L3 Exopolysaccharide" International Journal of Molecular Sciences 25, no. 13: 7185. https://doi.org/10.3390/ijms25137185

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop