Pretreatment of Melanoma Cells with Aqueous Ethanol Extract from Madhuca longifolia Bark Strongly Potentiates the Activity of a Low Dose of Dacarbazine
Abstract
:1. Introduction
2. Results
2.1. Analysis of Madhuca longifolia Extracts
2.2. Influence of the Extracts on Cell Viability
2.3. Influence of MLE on Cell Cycle
2.4. Induction of Apoptosis
2.5. Influence of MLE on Intracellular ROS Level
3. Discussion
4. Materials and Methods
4.1. Chemicals
4.2. Plant Material
4.3. Extraction
4.4. UHPLC-ESI-MS and MS/MS Analysis
4.5. Cell Culture
4.6. Cytotoxicity Assay
4.7. Flow Cytometry
4.8. Caspase 3 Acitvity
4.9. Combination Index Analysis
4.10. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Cell Line | Concentration (μg/mL) | Ratio | Combination Index (CI) | |
---|---|---|---|---|
MLE | Dacarbazine | |||
0.05 | 1 | 1:20 | 0.976 | |
1205-Lu | 0.10 | 1 | 1:10 | 0.654 |
0.50 | 1 | 1:2 | 0.892 | |
5.00 | 1 | 5:1 | 1.345 | |
0.05 | 1 | 1:20 | 0.954 | |
Me45 | 0.10 | 1 | 1:10 | 0.695 |
0.50 | 1 | 1:2 | 0.912 | |
5.00 | 1 | 5:1 | 1.327 |
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Środa-Pomianek, K.; Barycka, A.; Gleńsk, M.; Rajbhandari, M.; Skonieczna, M.; Palko-Łabuz, A.; Wesołowska, O. Pretreatment of Melanoma Cells with Aqueous Ethanol Extract from Madhuca longifolia Bark Strongly Potentiates the Activity of a Low Dose of Dacarbazine. Int. J. Mol. Sci. 2024, 25, 7220. https://doi.org/10.3390/ijms25137220
Środa-Pomianek K, Barycka A, Gleńsk M, Rajbhandari M, Skonieczna M, Palko-Łabuz A, Wesołowska O. Pretreatment of Melanoma Cells with Aqueous Ethanol Extract from Madhuca longifolia Bark Strongly Potentiates the Activity of a Low Dose of Dacarbazine. International Journal of Molecular Sciences. 2024; 25(13):7220. https://doi.org/10.3390/ijms25137220
Chicago/Turabian StyleŚroda-Pomianek, Kamila, Anna Barycka, Michał Gleńsk, Meena Rajbhandari, Magdalena Skonieczna, Anna Palko-Łabuz, and Olga Wesołowska. 2024. "Pretreatment of Melanoma Cells with Aqueous Ethanol Extract from Madhuca longifolia Bark Strongly Potentiates the Activity of a Low Dose of Dacarbazine" International Journal of Molecular Sciences 25, no. 13: 7220. https://doi.org/10.3390/ijms25137220