Sickle Cell Disease and Gut Health: The Influence of Intestinal Parasites and the Microbiome on Angolan Children
Abstract
:1. Introduction
2. Results
2.1. Prevalence of Parasitic Infections
2.2. Parasite Prevalence according to Residency
2.3. SCD Clinical Data and Parasitic Infections
2.4. Impact of Parasites on Gut Microbiome
3. Discussion
4. Materials and Methods
4.1. Population and Sample Collection
4.2. Clinical Analysis
4.3. Molecular Analysis
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Giardia (+) | Entamoeba (+) | Ascaris (+) | Enterobius (+) | |
---|---|---|---|---|
↓ Lactobacillus_uc | p = 0.010 | p = 0.031 | n.s. | p = 0.042 |
↓ Bifidobacterium_uc | n.s. | p < 0.001 | p = 0.001 | n.s. |
↓ Cuneatibacter | p = 0.002 | n.s. | p < 0.001 | p = 0.029 |
↓ Bacteroides uniformis | p = 0.025 | p = 0.017 | p = 0.021 | n.s. |
↓ Roseburia_uc | n.s. | p = 0.026 | p = 0.001 | p = 0.040 |
↓ Shuttleworthia_uc | p = 0.011 | p < 0.001 | p = 0.003 | n.s. |
Target Parasite | Molecular Target | Oligonucleotides | Sequence (5′-3′) | Melting Temperature | References |
---|---|---|---|---|---|
Entamoeba histolytica | SSU rRNA | FW | ATTGTCGTGGCATCCTAACTCA | 76.5 °C | [46] |
REV | GCGGACGGCTCATTATAACA | ||||
Hymenolepis nana | ITS1 | FW | CATTGTGTACCAAATTGATGATGAGTA | 79.5 °C | [47] |
REV | CAACTGACAGCATGTTTCGATATG | ||||
Strongyloides stercoralis | 18S | FW | GAATTCCAAGTAAACGTAAGTCATTAGC | 81.5 °C | [48] |
REV | TGCCTCTGGATATTGCTCAGTTC | ||||
Ascaris lumbricoides | ITS1 | FW | GTAATAGCAGTCGGCGGTTTCTT | [48] | |
REV | GCCCAACATGCCACCTATTC | ||||
Probe | FAM-TTGGCGGACAATTGCATGCGAT-BHQ1 | ||||
Giardia intestinalis | SSU rRNA | FW | GACGGCTCAGGACAACGGTT | [46] | |
REV | TTGCCAGCGGTGTCCG | ||||
Probe | FAM-CCCGCGGCGGTCCCTGCTAG-BHQ1 | ||||
Schistosoma mansoni | 90 bp fragment of a highly repetitive 121 bp sequence of S. mansoni | FW | CCGACCAACCGTTCTATGA | [49] | |
REV | CACGCTCTCGCAAATAATCTAAA | ||||
Probe | FAM-TCGTTGTATCTCCGAAACCACTGGACG-BHQ1 | ||||
Enterobius vermicularis | ITS1 | FW | CGGTGTAATTTTGTTGGTGTCTATG | [47] | |
REV | TGGCAGCATTGCAAACTAATG | ||||
Probe | FAM-TGTGCCAGTCAACGCCTAAACCGTC-BHQ1 | ||||
Trichuris trichiura | 18S | FW | TTGAAACGACTTGCTCATCAACTT | [50] | |
REV | CTGATTCTCCGTTAACCGTTGTC | ||||
Probe | FAM-CGATGGTACGCTACGTGCTTACCATGG-BHQ1 |
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Delgadinho, M.; Ginete, C.; Santos, B.; de Vasconcelos, J.N.; Arez, A.P.; Brito, M. Sickle Cell Disease and Gut Health: The Influence of Intestinal Parasites and the Microbiome on Angolan Children. Int. J. Mol. Sci. 2024, 25, 7258. https://doi.org/10.3390/ijms25137258
Delgadinho M, Ginete C, Santos B, de Vasconcelos JN, Arez AP, Brito M. Sickle Cell Disease and Gut Health: The Influence of Intestinal Parasites and the Microbiome on Angolan Children. International Journal of Molecular Sciences. 2024; 25(13):7258. https://doi.org/10.3390/ijms25137258
Chicago/Turabian StyleDelgadinho, Mariana, Catarina Ginete, Brígida Santos, Jocelyne Neto de Vasconcelos, Ana Paula Arez, and Miguel Brito. 2024. "Sickle Cell Disease and Gut Health: The Influence of Intestinal Parasites and the Microbiome on Angolan Children" International Journal of Molecular Sciences 25, no. 13: 7258. https://doi.org/10.3390/ijms25137258