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Volume 25
Issue 13
10.3390/ijms25137492
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Small and Large Extracellular Vesicles of Porcine Seminal Plasma Differ in Lipid Profile

by
Pablo Martínez-Díaz
1,
Ana Parra
1,
Christian M. Sanchez-López
2,3,
Josefina Casas
4,5,
Xiomara Lucas
1,
Antonio Marcilla
2,3,
Jordi Roca
1,*,† and
Isabel Barranco
1,†
1
Department of Medicine and Animal Surgery, Faculty of Veterinary Science, University of Murcia, 30100 Murcia, Spain
2
Àrea de Parasitologia, Departament de Farmàcia i Tecnologia Farmacèutica i Parasitologia, Universitat de València, 46100 Valencia, Spain
3
Joint Research Unit on Endocrinology, Nutrition and Clinical Dietetics, Health Research Institute La Fe, Universitat de València, 46100 Valencia, Spain
4
Research Unit on BioActive Molecules (RUBAM), Institute for Advanced Chemistry (IQAC-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain
5
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas (CIBEREHD), Instituto de Salud Carlos III, 28029 Madrid, Spain
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(13), 7492; https://doi.org/10.3390/ijms25137492
Submission received: 5 June 2024 / Revised: 3 July 2024 / Accepted: 5 July 2024 / Published: 8 July 2024
(This article belongs to the Special Issue New Progress in Extracellular Vesicles)
Versions Notes

Abstract

Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that remains poorly characterized. This study aimed to characterize the lipidomic profile of two subsets of differently sized sEVs, small (S-) and large (L-), isolated from porcine seminal plasma by size-exclusion chromatography and characterized by an orthogonal approach. High-performance liquid chromatography–high-resolution mass spectrometry was used for lipidomic analysis. A total of 157 lipid species from 14 lipid classes of 4 major categories (sphingolipids, glycerophospholipids, glycerolipids, and sterols) were identified. Qualitative differences were limited to two cholesteryl ester species present only in S-sEVs. L-sEVs had higher levels of all quantified lipid classes due to their larger membrane surface area. The distribution pattern was different, especially for sphingomyelins (more in S-sEVs) and ceramides (more in L-sEVs). In conclusion, this study reveals differences in the lipidomic profile of two subsets of porcine sEVs, suggesting that they differ in biogenesis and functionality.
Keywords: extracellular vesicles; lipidomics; porcine; seminal plasma; subsets extracellular vesicles; lipidomics; porcine; seminal plasma; subsets

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MDPI and ACS Style

Martínez-Díaz, P.; Parra, A.; Sanchez-López, C.M.; Casas, J.; Lucas, X.; Marcilla, A.; Roca, J.; Barranco, I. Small and Large Extracellular Vesicles of Porcine Seminal Plasma Differ in Lipid Profile. Int. J. Mol. Sci. 2024, 25, 7492. https://doi.org/10.3390/ijms25137492

AMA Style

Martínez-Díaz P, Parra A, Sanchez-López CM, Casas J, Lucas X, Marcilla A, Roca J, Barranco I. Small and Large Extracellular Vesicles of Porcine Seminal Plasma Differ in Lipid Profile. International Journal of Molecular Sciences. 2024; 25(13):7492. https://doi.org/10.3390/ijms25137492

Chicago/Turabian Style

Martínez-Díaz, Pablo, Ana Parra, Christian M. Sanchez-López, Josefina Casas, Xiomara Lucas, Antonio Marcilla, Jordi Roca, and Isabel Barranco. 2024. "Small and Large Extracellular Vesicles of Porcine Seminal Plasma Differ in Lipid Profile" International Journal of Molecular Sciences 25, no. 13: 7492. https://doi.org/10.3390/ijms25137492

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