This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Open AccessArticle
Overexpression of BnaA10.WRKY75 Decreases Cadmium and Salt Tolerance via Increasing ROS Accumulation in Arabidopsis and Brassica napus L
by
Xiaoke Ping
Xiaoke Ping
Qianjun Ye
Qianjun Ye
Mei Yan
Mei Yan
Jia Wang
Jia Wang
Taiyuan Zhang
Taiyuan Zhang
Sheng Chen
Sheng Chen 
,
Kadambot H. M. Siddique
Kadambot H. M. Siddique
Wallace A. Cowling
Wallace A. Cowling
Jiana Li
Jiana Li
Prof. Jiana Li is a second-level professor and doctoral
supervisor at Southwest Agricultural He has [...]
Prof. Jiana Li is a second-level professor and doctoral
supervisor at Southwest Agricultural University. He has been working at the
university since 1985 as a lecturer, associate professor, dean and executive
vice minister of the Ministry of Agriculture. He studied for his PhD degree at Sichuan University,
majoring in botany, from 1995 to 1998. From May to October 2001, he was a visiting scholar at the
Canadian Department of Agriculture Saskatoon AAFC. In addition, he holds
many social and academic roles, is an executive member of the Oil Crop
Professional Committee of the Chinese Crop Society, a member of the Plant and
Genome Professional Committee of the Chinese Genetic Society, an expert
committee member of the China High-Quality Agricultural Product Development
Service Association, a member of the National Rapeseed Variety Comparative
Experiment Expert Group, and so on. His research direction is principles and methods
of crop breeding, plant cell and molecular genetics, and rapeseed science.
Liezhao Liu
Liezhao Liu
Prof. Liezhao Liu is a lecturer at the Southwest University, School of Agriculture
and He has at He [...]
Prof. Liezhao Liu is a lecturer at the Southwest University, School of Agriculture
and Biotechnology. He has worked at the university since 2004. He studied for his
PhD degree at the Southwest Agricultural University, majoring in genetic
breeding, from 2000 to 2003. In 2006, he studied at the Northeastern University
of Wales (UK). From 2008 to 2011, he attended Giessen University (Germany) for a
postdoctoral fellowship. He has led and participated in many scientific research projects,
such as the localization of cellulose components in the grains and stems of
Brassica napus, the construction of a testing network in the upper reaches
of the Yangtze River and the transcription factor BnaA9.NF-YA7 in Brassica
napus research on drought tolerance function and molecular mechanism. His
research direction is research on the utilization of lignin metabolism-related
genes in rapeseed, functional analysis of stress biology-related genes in
rapeseed and mapping and functional analysis of agronomic trait genes in
rapeseed.
1
College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
2
The UWA Institute of Agriculture, The University of Western Australia, Perth, WA 6000, Australia
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this paper as first authors.
Int. J. Mol. Sci. 2024, 25(14), 8002; https://doi.org/10.3390/ijms25148002 (registering DOI)
Submission received: 4 July 2024
/
Revised: 17 July 2024
/
Accepted: 20 July 2024
/
Published: 22 July 2024
Abstract
Soil is indispensable for agricultural production but has been seriously polluted by cadmium and salt in recent years. Many crops are suffering from this, including rapeseed, the third largest global oilseed crop. However, genes simultaneously related to both cadmium and salt stress have not been extensively reported yet. In this study, BnaA10.WRKY75 was screened from previous RNA-seq data related to cadmium and salt stress and further analyses including sequence comparison, GUS staining, transformation and qRT-PCR were conducted to confirm its function. GUS staining and qRT-PCR results indicated BnaA10.WRKY75 was induced by CdCl2 and NaCl treatment. Sequence analysis suggested BnaA10.WRKY75 belongs to Group IIc of the WRKY gene family and transient expression assay showed it was a nuclear localized transcription factor. BnaA10.WRKY75-overexpressing Arabidopsis and rapeseed plants accumulated more H2O2 and O2− and were more sensitive to CdCl2 and NaCl treatment compared with untransformed plants, which may be caused by the downregulation of BnaC03.CAT2. Our study reported that BnaA10.WRKY75 increases sensitivity to cadmium and salt stress by disrupting the balance of reactive oxygen species both in Arabidopsis and rapeseed. The results support the further understanding of the mechanisms underlying cadmium and salt tolerance and provide BnaA10.WRKY75 as a valuable gene for rapeseed abiotic stress breeding.
Share and Cite
MDPI and ACS Style
Ping, X.; Ye, Q.; Yan, M.; Wang, J.; Zhang, T.; Chen, S.; Siddique, K.H.M.; Cowling, W.A.; Li, J.; Liu, L.
Overexpression of BnaA10.WRKY75 Decreases Cadmium and Salt Tolerance via Increasing ROS Accumulation in Arabidopsis and Brassica napus L. Int. J. Mol. Sci. 2024, 25, 8002.
https://doi.org/10.3390/ijms25148002
AMA Style
Ping X, Ye Q, Yan M, Wang J, Zhang T, Chen S, Siddique KHM, Cowling WA, Li J, Liu L.
Overexpression of BnaA10.WRKY75 Decreases Cadmium and Salt Tolerance via Increasing ROS Accumulation in Arabidopsis and Brassica napus L. International Journal of Molecular Sciences. 2024; 25(14):8002.
https://doi.org/10.3390/ijms25148002
Chicago/Turabian Style
Ping, Xiaoke, Qianjun Ye, Mei Yan, Jia Wang, Taiyuan Zhang, Sheng Chen, Kadambot H. M. Siddique, Wallace A. Cowling, Jiana Li, and Liezhao Liu.
2024. "Overexpression of BnaA10.WRKY75 Decreases Cadmium and Salt Tolerance via Increasing ROS Accumulation in Arabidopsis and Brassica napus L" International Journal of Molecular Sciences 25, no. 14: 8002.
https://doi.org/10.3390/ijms25148002
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details
here.
Article Metrics
Article metric data becomes available approximately 24 hours after publication online.
