Evaluation of the Potential Cytoprotective Effect of Melatonin in Comparison with Vitamin E and Trolox against Cd2+-Induced Toxicity in SH-SY5Y, HCT 116, and HepG2 Cell Lines
Abstract
:1. Introduction
2. Results
2.1. Effect Cd2+, MLT, Vit E, and Trolox on Cell Viability
2.2. Effect of MLT, VitE, and Trolox on Cd2+-Induced Cytotoxicity
2.3. ROS Scavenging Effects of MLT, VitE, and Trolox against Cd2+- and H2O2-Induced Oxidative Stress
3. Discussion
3.1. Effect of Cd, MLT, VitE, and Trolox on Cell Viability
3.2. Effect of MLT, VitE, and Trolox on Cd-Induced Cytotoxicity
3.3. ROS Scavenging Effects of MLT, VitE, and Trolox on Cd-Induced Oxidative Stress
4. Materials and Methods
4.1. Cytotoxicity and Cytoprotective Assays In Vitro
4.1.1. Cell Culture
4.1.2. Preparation of Heavy Metal Solutions and Antioxidant Agents
4.1.3. Cytoprotective and Proliferative Effects
4.1.4. Cell Viability Evaluation by the Colorimetric MTT Assay
4.1.5. Measurement of Intracellular ROS Production
4.2. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Mallamaci, R.; Barbarossa, A.; Carrieri, A.; Meleleo, D.; Carocci, A. Evaluation of the Potential Cytoprotective Effect of Melatonin in Comparison with Vitamin E and Trolox against Cd2+-Induced Toxicity in SH-SY5Y, HCT 116, and HepG2 Cell Lines. Int. J. Mol. Sci. 2024, 25, 8055. https://doi.org/10.3390/ijms25158055
Mallamaci R, Barbarossa A, Carrieri A, Meleleo D, Carocci A. Evaluation of the Potential Cytoprotective Effect of Melatonin in Comparison with Vitamin E and Trolox against Cd2+-Induced Toxicity in SH-SY5Y, HCT 116, and HepG2 Cell Lines. International Journal of Molecular Sciences. 2024; 25(15):8055. https://doi.org/10.3390/ijms25158055
Chicago/Turabian StyleMallamaci, Rosanna, Alexia Barbarossa, Antonio Carrieri, Daniela Meleleo, and Alessia Carocci. 2024. "Evaluation of the Potential Cytoprotective Effect of Melatonin in Comparison with Vitamin E and Trolox against Cd2+-Induced Toxicity in SH-SY5Y, HCT 116, and HepG2 Cell Lines" International Journal of Molecular Sciences 25, no. 15: 8055. https://doi.org/10.3390/ijms25158055