Moderate Aerobic Exercise Induces Homeostatic IgA Generation in Senile Mice
Abstract
:1. Introduction
2. Results
2.1. Exercise Increased Total B Cells in Senile Mice
2.2. Exercise Increased the IgA+ B Cells in Senile Mice
2.3. Exercise Increased the α-Chain and APRIL mRNA in Adult and Senile Mice
2.4. Factors Related to B Cells Maturation and Differentiation
2.5. Exercise Suppressed Parameters of pIgR for IgA-Transcytosis
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Experimental Design
4.3. Moderate Aerobic Exercise Protocol
4.4. Sampling
4.5. Lymphoid Sub-Populations in Lamina Propria by Cytometry
4.6. Relative Expression of mRNA Assay
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gen | Sequence 5′→3′ | ID | |
---|---|---|---|
Forward | Reverse | ||
TSLP | cag ctt gtc tcc tga aaa tcg | aaa tgt ttt gtc ggg gag tg | NM_021367.2 |
BAFF | atg cgg aag gca gat tga | tgc atc ttt tgc tac cct ga | NM_001347309.1 |
APRIL | agc tgg gca ctg agc ttt ac | aag ttg gcc tcg aac tca tc | NM_023517.2 |
iNOS | ctt ttc cta tgg ggc aaa aa | ctg gaa ctc tgg gct gtc a | NM_010927.4 |
RDH11 | tgt act tgg tca cgc caa aa | ccg gga agc tga aca tta ga | NM_021557.5 |
TGF-β1 | tgg agc aac atg tgg aac tc | gtc agc agc cgg tta cca | NM_011577.2 |
IL-6 | gct acc aaa ctg gat ata atc agg a | cca ggt agc tat ggt act cca gaa | NM_031168.2 |
CBFa3 | gct ctc tca gca cca cga g | tca ggt ctg agg agc ctt g | NM_019732.2 |
USF-1 | tca aga ggt ggg aaa gga tg | cat tgg gcc ccc ttc tac | NM_001305676.1 |
Pax5 | cct ggg agt gaa ttt tct gg | tgg gga acc tcc aag aat c | NM_008782.2 |
GAPDH | aag agg gat gct gcc ctt ac | cca ttt tgt cta cgg gac ga | NM_001289726.1 |
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Hernández-Urbán, A.J.; Drago-Serrano, M.-E.; Reséndiz-Albor, A.A.; Sierra-Ramírez, J.A.; Guzmán-Mejía, F.; Oros-Pantoja, R.; Godínez-Victoria, M. Moderate Aerobic Exercise Induces Homeostatic IgA Generation in Senile Mice. Int. J. Mol. Sci. 2024, 25, 8200. https://doi.org/10.3390/ijms25158200
Hernández-Urbán AJ, Drago-Serrano M-E, Reséndiz-Albor AA, Sierra-Ramírez JA, Guzmán-Mejía F, Oros-Pantoja R, Godínez-Victoria M. Moderate Aerobic Exercise Induces Homeostatic IgA Generation in Senile Mice. International Journal of Molecular Sciences. 2024; 25(15):8200. https://doi.org/10.3390/ijms25158200
Chicago/Turabian StyleHernández-Urbán, Angel J., Maria-Elisa Drago-Serrano, Aldo A. Reséndiz-Albor, José A. Sierra-Ramírez, Fabiola Guzmán-Mejía, Rigoberto Oros-Pantoja, and Marycarmen Godínez-Victoria. 2024. "Moderate Aerobic Exercise Induces Homeostatic IgA Generation in Senile Mice" International Journal of Molecular Sciences 25, no. 15: 8200. https://doi.org/10.3390/ijms25158200