This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Open AccessArticle
Transcriptomic Analysis of the Molecular Mechanism Potential of Grafting—Enhancing the Ability of Oriental Melon to Tolerate Low-Nitrogen Stress
by
Yulei Zhu
Yulei Zhu 1,2,3,†,
Ziqing Sun
Ziqing Sun 1,2,3,†,
Hongxi Wu
Hongxi Wu 1,2,3,
Caifeng Cui
Caifeng Cui 1,2,3,
Sida Meng
Sida Meng 1,2,3,4,* and
Chuanqiang Xu
Chuanqiang Xu 1,2,3,4,*
1
College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
2
Key Laboratory of Protected Horticulture (Ministry of Education), Shenyang Agricultural University, Shenyang 110866, China
3
Modern Protected Horticultural Engineering & Technology Center, Shenyang Agricultural University, Shenyang 110866, China
4
Key Laboratory of Horticultural Equipment (Ministry of Agriculture and Rural Affairs), Shenyang Agricultural University, Shenyang 110866, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(15), 8227; https://doi.org/10.3390/ijms25158227 (registering DOI)
Submission received: 25 May 2024
/
Revised: 25 July 2024
/
Accepted: 26 July 2024
/
Published: 27 July 2024
Abstract
Nitrogen is the primary nutrient for plants. Low nitrogen generally affects plant growth and fruit quality. Melon, as an economic crop, is highly dependent on nitrogen. However, the response mechanism of its self-rooted and grafted seedlings to low-nitrogen stress has not been reported previously. Therefore, in this study, we analyzed the transcriptional differences between self-rooted and grafted seedlings under low-nitrogen stress using fluorescence characterization and RNA-Seq analysis. It was shown that low-nitrogen stress significantly inhibited the fluorescence characteristics of melon self-rooted seedlings. Analysis of differentially expressed genes showed that the synthesis of genes related to hormone signaling, such as auxin and brassinolide, was delayed under low-nitrogen stress. Oxidative stress response, involved in carbon and nitrogen metabolism, and secondary metabolite-related differentially expressed genes (DEGs) were significantly down-regulated. It can be seen that low-nitrogen stress causes changes in many hormonal signals in plants, and grafting can alleviate the damage caused by low-nitrogen stress on plants, ameliorate the adverse effects of nitrogen stress on plants, and help them better cope with environmental stresses.
Share and Cite
MDPI and ACS Style
Zhu, Y.; Sun, Z.; Wu, H.; Cui, C.; Meng, S.; Xu, C.
Transcriptomic Analysis of the Molecular Mechanism Potential of Grafting—Enhancing the Ability of Oriental Melon to Tolerate Low-Nitrogen Stress. Int. J. Mol. Sci. 2024, 25, 8227.
https://doi.org/10.3390/ijms25158227
AMA Style
Zhu Y, Sun Z, Wu H, Cui C, Meng S, Xu C.
Transcriptomic Analysis of the Molecular Mechanism Potential of Grafting—Enhancing the Ability of Oriental Melon to Tolerate Low-Nitrogen Stress. International Journal of Molecular Sciences. 2024; 25(15):8227.
https://doi.org/10.3390/ijms25158227
Chicago/Turabian Style
Zhu, Yulei, Ziqing Sun, Hongxi Wu, Caifeng Cui, Sida Meng, and Chuanqiang Xu.
2024. "Transcriptomic Analysis of the Molecular Mechanism Potential of Grafting—Enhancing the Ability of Oriental Melon to Tolerate Low-Nitrogen Stress" International Journal of Molecular Sciences 25, no. 15: 8227.
https://doi.org/10.3390/ijms25158227
Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details
here.
Article Metrics
Article metric data becomes available approximately 24 hours after publication online.