A Transcriptomic Analysis of Bottle Gourd-Type Rootstock Roots Identifies Novel Transcription Factors Responsive to Low Root Zone Temperature Stress
Abstract
:1. Introduction
2. Results
2.1. LRT Stress Has a Major Impact on the Bottle Gourd Root
2.2. LRT Affects Bottle Gourd Roots’ Physiological Traits
2.3. Transcriptomic Analysis Reveals DEGs in Response to LRT Stress
2.4. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Enrichment Analysis among DEGs Involved in Translation and the Stress Response
2.5. TF Families Respond to LRT Differentially
2.6. qRT-PCR Analysis Validates the Expression Patterns of DEGs
3. Discussion
3.1. LRT Stress Affected the Phenotype and Physiology of Bottle Gourd Roots
3.2. Dynamic Changes in ROS Levels Occurred under LRT Conditions
3.3. Differentially Expressed TFs of the Bottle Gourd Root in Response to LRT Stress
4. Materials and Methods
4.1. Plant Materials, 15 °C LRT Treatment and Sample Preparation
4.2. Cell Viability Staining
4.3. Determination of Relative Electrolyte Leakage
4.4. Determination of Malondialdehyde (MDA) and Peroxide Enzyme Activity
4.5. Total RNA Extraction
4.6. Library Construction and RNA-Seq Data Analysis
4.7. Quantitative Real-Time PCR (qRT-PCR) for RNA-Seq Validation
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, J.; Zhang, M.; Xu, J.; Yao, X.; Lou, L.; Hou, Q.; Zhu, L.; Yang, X.; Liu, G.; Xu, J. A Transcriptomic Analysis of Bottle Gourd-Type Rootstock Roots Identifies Novel Transcription Factors Responsive to Low Root Zone Temperature Stress. Int. J. Mol. Sci. 2024, 25, 8288. https://doi.org/10.3390/ijms25158288
Liu J, Zhang M, Xu J, Yao X, Lou L, Hou Q, Zhu L, Yang X, Liu G, Xu J. A Transcriptomic Analysis of Bottle Gourd-Type Rootstock Roots Identifies Novel Transcription Factors Responsive to Low Root Zone Temperature Stress. International Journal of Molecular Sciences. 2024; 25(15):8288. https://doi.org/10.3390/ijms25158288
Chicago/Turabian StyleLiu, Jinqiu, Man Zhang, Jian Xu, Xiefeng Yao, Lina Lou, Qian Hou, Lingli Zhu, Xingping Yang, Guang Liu, and Jinhua Xu. 2024. "A Transcriptomic Analysis of Bottle Gourd-Type Rootstock Roots Identifies Novel Transcription Factors Responsive to Low Root Zone Temperature Stress" International Journal of Molecular Sciences 25, no. 15: 8288. https://doi.org/10.3390/ijms25158288