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Open AccessArticle
Carbon Source and Substrate Surface Affect Biofilm Formation by the Plant-Associated Bacterium Pseudomonas donghuensis P482
by
Magdalena Rajewska
Magdalena Rajewska 1,*,
Tomasz Maciąg
Tomasz Maciąg 2
,
Magdalena Narajczyk
Magdalena Narajczyk 3
and
Sylwia Jafra
Sylwia Jafra 1
1
Laboratory of Plant Microbiology, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, Abrahama 58, 80-307 Gdansk, Poland
2
Institute of Biology, Department of Botany, Warsaw University of Life Sciences, Nowoursynowska 159, 02-776 Warsaw, Poland
3
Laboratory of Electron Microscopy, Faculty of Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(15), 8351; https://doi.org/10.3390/ijms25158351 (registering DOI)
Submission received: 5 July 2024
/
Revised: 25 July 2024
/
Accepted: 26 July 2024
/
Published: 30 July 2024
Abstract
The ability of bacteria to colonize diverse environmental niches is often linked to their competence in biofilm formation. It depends on the individual characteristics of a strain, the nature of the colonized surface (abiotic or biotic), or the availability of certain nutrients. Pseudomonas donghuensis P482 efficiently colonizes the rhizosphere of various plant hosts, but a connection between plant tissue colonization and the biofilm formation ability of this strain has not yet been established. We demonstrate here that the potential of P482 to form biofilms on abiotic surfaces and the structural characteristics of the biofilm are influenced by the carbon source available to the bacterium, with glycerol promoting the process. Also, the type of substratum, polystyrene or glass, impacts the ability of P482 to attach to the surface. Moreover, P482 mutants in genes associated with motility or chemotaxis, the synthesis of polysaccharides, and encoding proteases or regulatory factors, which affect biofilm formation on glass, were fully capable of colonizing the root tissue of both tomato and maize hosts. Investigating the role of cellular factors in biofilm formation using these plant-associated bacteria shows that the ability of bacteria to form biofilm on abiotic surfaces does not necessarily mirror its ability to colonize plant tissues. Our research provides a broader perspective on the adaptation of these bacteria to various environments.
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MDPI and ACS Style
Rajewska, M.; Maciąg, T.; Narajczyk, M.; Jafra, S.
Carbon Source and Substrate Surface Affect Biofilm Formation by the Plant-Associated Bacterium Pseudomonas donghuensis P482. Int. J. Mol. Sci. 2024, 25, 8351.
https://doi.org/10.3390/ijms25158351
AMA Style
Rajewska M, Maciąg T, Narajczyk M, Jafra S.
Carbon Source and Substrate Surface Affect Biofilm Formation by the Plant-Associated Bacterium Pseudomonas donghuensis P482. International Journal of Molecular Sciences. 2024; 25(15):8351.
https://doi.org/10.3390/ijms25158351
Chicago/Turabian Style
Rajewska, Magdalena, Tomasz Maciąg, Magdalena Narajczyk, and Sylwia Jafra.
2024. "Carbon Source and Substrate Surface Affect Biofilm Formation by the Plant-Associated Bacterium Pseudomonas donghuensis P482" International Journal of Molecular Sciences 25, no. 15: 8351.
https://doi.org/10.3390/ijms25158351
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