Identification of Oxidative Stress-Related Biomarkers for Pain–Depression Comorbidity Based on Bioinformatics
Abstract
:1. Introduction
2. Results
2.1. Identification of DEGs and DEOSGs
2.2. Enrichment Analysis of KEGG Pathways and GO Functions for DEOSGs
2.3. Screening of Pathway Enrichments by GSEA
2.4. Construction of the WGCNA Network and Identification of Key Genes
2.5. Enrichment Analysis of Key Genes
2.6. Construction of the PPI Network and Screening of Hub Genes
2.7. Correlation Analysis between Hub Genes and Immune Cells
2.8. Validation of Hub Gene Expression
2.9. Identification of Transcription Factors, microRNAs and Drugs Regulating Key Genes
3. Discussion
4. Materials and Methods
4.1. Data Collection
4.2. Screening of DEGs
4.3. Functional Enrichment Analysis for DEOSGs
4.4. Gene Set-Enrichment Analysis
4.5. Weighted Gene Co-Expression Network Analysis
4.6. Identification of Key Genes
4.7. Functional Enrichment Analysis for Key Genes
4.8. PPI Network Construction and Topology Network Interaction Analysis
4.9. Immune Cell Infiltration Analysis
4.10. Exploration of miRNAs and TFs Regulating Hub Genes
4.11. Identification of Potentially Effective Drugs Targeting Hub Genes
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zhang, T.; Geng, M.; Li, X.; Gu, Y.; Zhao, W.; Ning, Q.; Zhao, Z.; Wang, L.; Zhang, H.; Zhang, F. Identification of Oxidative Stress-Related Biomarkers for Pain–Depression Comorbidity Based on Bioinformatics. Int. J. Mol. Sci. 2024, 25, 8353. https://doi.org/10.3390/ijms25158353
Zhang T, Geng M, Li X, Gu Y, Zhao W, Ning Q, Zhao Z, Wang L, Zhang H, Zhang F. Identification of Oxidative Stress-Related Biomarkers for Pain–Depression Comorbidity Based on Bioinformatics. International Journal of Molecular Sciences. 2024; 25(15):8353. https://doi.org/10.3390/ijms25158353
Chicago/Turabian StyleZhang, Tianyun, Menglu Geng, Xiaoke Li, Yulin Gu, Wenjing Zhao, Qi Ning, Zijie Zhao, Lei Wang, Huaxing Zhang, and Fan Zhang. 2024. "Identification of Oxidative Stress-Related Biomarkers for Pain–Depression Comorbidity Based on Bioinformatics" International Journal of Molecular Sciences 25, no. 15: 8353. https://doi.org/10.3390/ijms25158353