An In-Depth Approach to the Associations between MicroRNAs and Viral Load in Patients with Chronic Hepatitis B—A Systematic Review and Meta-Analysis
Abstract
:1. Introduction
2. Material and Methods
2.1. The Article Extraction Process
2.2. The Selection Method
2.3. Data Acquisition and Quality Assessment
2.4. A Bioinformatics Analysis on the Retrieved microRNAs
2.5. Statistical Methods
3. Results
3.1. The Article Selection Scheme
3.2. Main Article Data
3.3. Meta-Correlation of Spearman Results
3.4. Meta-Correlation of Pearson Results
3.5. Bioinformatics Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Article Reference | Study Site | Article Type | Patient Counts | Patient Average Age | Identified microRNA | Detection Method | Correlation Assessment (between microRNAs and Viral Loads) | NOS Score * |
---|---|---|---|---|---|---|---|---|
Yu et al., 2015 [66] | China | Cross-sectional | CHB group: 115 HC: 20 | CHB group: 52.84 ± 3.82 HC: 49.99 ± 5.16 | miR-210 (serum samples) | RT-PCR | Spearman: r = 0.525 p < 0.001 | 7 |
van der Ree et al., 2017 [67] | Nether-lands | Cohort | CHB group: 92 | Cohort 1: HBeAg positives- 35 ± 10 HBeAg negatives- 42 ± 12 Cohort 2: HBeAg positives- 35 ± 9 HBeAg negatives- 44 ± 10 | miR-122-5p miR-125b-5p miR-192-5p miR-193b-3p miR-194-5p miR-200a-3p miR-204-5p miR-29a-5p (plasma samples) | RT-PCR | Pearson: miR-122-5p- r = 0.468 p = 0.01 miR-125b-5p- r = 0.599 p < 0.01 miR-192-5p- r = 0.483 p < 0.01 miR-193b-3p- r = 0.422 p < 0.01 miR-194-5p- r = 0.585 p < 0.01 miR-200a-3p- r = 0.130 p > 0.05 miR-204-5p- r = −0.003 p > 0.05 miR-29a-5p- r = 0.228 p < 0.05 | 8 |
Zhou et al., 2018 [68] | China | Cohort | CHB group HBeAg negative treated with NA: 66 | CHB group: 31.55 ± 10 | miR-125b (serum samples) | RT-PCR | Spearman: r = 0.353 p = 0.004 (baseline measurement) | 9 |
Liu. et al., 2021 [69] | China | Cross-sectional | HBV group: 62 Controls: 11 | HBV carrier group: 30.29 ± 11.19 CHB group: 38 ± 10.81 Cirrhosis group: 43.29 ± 6.28 Control group: 27.45 ± 6.2 | miR-122 (serum samples) | RT-PCR | Spearman: r = 0.354 p = 0.005 | 7 |
Tan et al., 2021 [70] | China | Cohort | CHB group with HBeAg seroconversion after treatment: 36 | CHB responsive group to treatment: 33.72 ± 10.47 CHB non-responsive group to treatment: 33.78 ± 8.72 | miR-122-5p miR-320a-3p (serum samples) | Sequencing technique | Spearman: miR-122-5p- r = 0.438 p = 0.008 Pearson: miR-320a-3p- r = −0.366 p = 0.028 | 8 |
Li et al., 2022 [71] | China | Cross-sectional+ in vitro analysis | CHB group: 109 HC group: 20 | - | miR-192-3p (serum samples) | RT-PCR | Spearman: r = 0.37 p = 0.0002 | 7 |
Nagura et al., 2022 [72] | Japan | Cohort | CHB group: 61 | CHB group: 36.06 ± 8.35 | miR-192-5p (serum samples) | RT-PCR | Pearson: baseline- r = 0.484 p < 0.001 Pearson: In week 24- r = 0.655 p < 0.001 | 9 |
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Manea, M.; Mărunțelu, I.; Constantinescu, I. An In-Depth Approach to the Associations between MicroRNAs and Viral Load in Patients with Chronic Hepatitis B—A Systematic Review and Meta-Analysis. Int. J. Mol. Sci. 2024, 25, 8410. https://doi.org/10.3390/ijms25158410
Manea M, Mărunțelu I, Constantinescu I. An In-Depth Approach to the Associations between MicroRNAs and Viral Load in Patients with Chronic Hepatitis B—A Systematic Review and Meta-Analysis. International Journal of Molecular Sciences. 2024; 25(15):8410. https://doi.org/10.3390/ijms25158410
Chicago/Turabian StyleManea, Marina, Ion Mărunțelu, and Ileana Constantinescu. 2024. "An In-Depth Approach to the Associations between MicroRNAs and Viral Load in Patients with Chronic Hepatitis B—A Systematic Review and Meta-Analysis" International Journal of Molecular Sciences 25, no. 15: 8410. https://doi.org/10.3390/ijms25158410