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Volume 25
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10.3390/ijms25158438
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Communication

Immunohistochemistry Screening of Different Tyrosine Kinase Receptors in Canine Solid Tumors—Part I: Proposal of a Receptor Panel to Predict Therapies

by
Denner Santos Dos Anjos
1,
Patrick Antônio Sonaglio Civa
2,
Juliana Werner
3,
Igor Simões Tiagua Vicente
4,5 and
Carlos Eduardo Fonseca-Alves
1,4,5,*
1
Department of Veterinary Surgery and Animal Reproduction, Universidade Estadual Paulista (UNESP), Botucatu 18618-681, Brazil
2
Medicalvet Veterinary Clinic, Chapecó 89802-272, Brazil
3
Werner and Werner Laboratory, Curitiba 80540-160, Brazil
4
VetPrecision Laboratory, Botucatu 18608-970, Brazil
5
Institute of Veterinary Oncology, IOVET, São Paulo 05027020, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2024, 25(15), 8438; https://doi.org/10.3390/ijms25158438
Submission received: 15 May 2024 / Revised: 23 July 2024 / Accepted: 30 July 2024 / Published: 2 August 2024
(This article belongs to the Special Issue Oncogenic Signaling of Growth Factor Receptors in Cancer 2.0: Mechanisms and Therapeutic Opportunities)
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Abstract

The use of tyrosine kinase inhibitors (TKI) has been growing in veterinary oncology and in the past few years several TKI have been tested in dogs. However, different from human medicine, we lack strategies to select patients to be treated with each TKI. Therefore, this study aimed to screen different tumor subtypes regarding TKI target immunoexpression as a predictor strategy to personalize the canine cancer treatment. It included 18 prostatic carcinomas, 36 soft tissue sarcomas, 20 mammary gland tumors, 6 urothelial bladder carcinomas, and 7 tumors from the endocrine system. A total of 87 patients with paraffin blocks were used to perform immunohistochemistry (IHC) of human epidermal growth factor receptor 2 (HER-2), epidermal growth factor receptors 1 (EGFR1), vascular endothelial growth factor receptor 2 (VEGFR-2), platelet derived growth factor receptor beta (PDGFR-β), c-KIT, and extracellular signal-regulated kinase 1/2 (ERK1/ERK2). The immunohistochemical screening revealed a heterogeneous protein expression among histological types with mesenchymal tumors showing the lowest expression level and carcinomas the highest expression. We have demonstrated by IHC screening that HER2, EGFR1, VEGFR-2, PDGFR-β and ERK1/ERK2 are commonly overexpressed in dogs with different carcinomas, and KIT expression is considered relatively low in the analyzed samples.
Keywords: dogs; epidermal growth factor; immunohistochemistry; platelet-derived growth factor receptor; receptor tyrosine kinase; target therapy; vascular endothelial growth factor dogs; epidermal growth factor; immunohistochemistry; platelet-derived growth factor receptor; receptor tyrosine kinase; target therapy; vascular endothelial growth factor

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MDPI and ACS Style

Dos Anjos, D.S.; Civa, P.A.S.; Werner, J.; Vicente, I.S.T.; Fonseca-Alves, C.E. Immunohistochemistry Screening of Different Tyrosine Kinase Receptors in Canine Solid Tumors—Part I: Proposal of a Receptor Panel to Predict Therapies. Int. J. Mol. Sci. 2024, 25, 8438. https://doi.org/10.3390/ijms25158438

AMA Style

Dos Anjos DS, Civa PAS, Werner J, Vicente IST, Fonseca-Alves CE. Immunohistochemistry Screening of Different Tyrosine Kinase Receptors in Canine Solid Tumors—Part I: Proposal of a Receptor Panel to Predict Therapies. International Journal of Molecular Sciences. 2024; 25(15):8438. https://doi.org/10.3390/ijms25158438

Chicago/Turabian Style

Dos Anjos, Denner Santos, Patrick Antônio Sonaglio Civa, Juliana Werner, Igor Simões Tiagua Vicente, and Carlos Eduardo Fonseca-Alves. 2024. "Immunohistochemistry Screening of Different Tyrosine Kinase Receptors in Canine Solid Tumors—Part I: Proposal of a Receptor Panel to Predict Therapies" International Journal of Molecular Sciences 25, no. 15: 8438. https://doi.org/10.3390/ijms25158438

APA Style

Dos Anjos, D. S., Civa, P. A. S., Werner, J., Vicente, I. S. T., & Fonseca-Alves, C. E. (2024). Immunohistochemistry Screening of Different Tyrosine Kinase Receptors in Canine Solid Tumors—Part I: Proposal of a Receptor Panel to Predict Therapies. International Journal of Molecular Sciences, 25(15), 8438. https://doi.org/10.3390/ijms25158438

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