Effects of Ginsenoside Rb1 on the Crosstalk between Intestinal Stem Cells and Microbiota in a Simulated Weightlessness Mouse Model
Abstract
:1. Introduction
2. Results
2.1. Rb1 Enhanced Intestinal Barrier Function in HU Mice
2.2. Rb1 Promoted ISC Homeostasis in HU Mice
2.3. Rb1 Improved the Niche Function of ISCs
2.4. Rb1 Attenuated Gut Dysbiosis in HU Mice
2.5. Rb1 Protected HU-Induced ISC Injury via Microbiota and/or Microbiota-Associated Metabolites
2.6. Rb1 Derivative Promoted the Growth of Akkermansia and Bifidobacterium
3. Discussion
4. Materials and Methods
4.1. Humane Care of Animals
4.2. Hindlimb Unloading and Rb1 Treatment
4.3. Co-Housing Experimental Design
4.4. Histology and Immunohistochemistry
4.5. Enrichment of Small Intestinal Epithelial Cells
4.6. ELISA
4.7. Flow Cytometry
4.8. RNA Isolation and Real-Time PCR
4.9. RNA Sequencing and Analysis
4.10. Western Blotting
4.11. 16s rRNA Sequencing and Data Analysis
4.12. Bacterial Growth and Measurements
4.13. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Zong, B.; Wang, J.; Wang, K.; Hao, J.; Han, J.-Y.; Jin, R.; Ge, Q. Effects of Ginsenoside Rb1 on the Crosstalk between Intestinal Stem Cells and Microbiota in a Simulated Weightlessness Mouse Model. Int. J. Mol. Sci. 2024, 25, 8769. https://doi.org/10.3390/ijms25168769
Zong B, Wang J, Wang K, Hao J, Han J-Y, Jin R, Ge Q. Effects of Ginsenoside Rb1 on the Crosstalk between Intestinal Stem Cells and Microbiota in a Simulated Weightlessness Mouse Model. International Journal of Molecular Sciences. 2024; 25(16):8769. https://doi.org/10.3390/ijms25168769
Chicago/Turabian StyleZong, Beibei, Jingyi Wang, Kai Wang, Jie Hao, Jing-Yan Han, Rong Jin, and Qing Ge. 2024. "Effects of Ginsenoside Rb1 on the Crosstalk between Intestinal Stem Cells and Microbiota in a Simulated Weightlessness Mouse Model" International Journal of Molecular Sciences 25, no. 16: 8769. https://doi.org/10.3390/ijms25168769