Antibiotics Trigger Host Innate Immune Response via Microbiota–Brain Communication in C. elegans
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Screening to Identify E. coli Genes Whose Inactivation Activates Detoxification and Immune Response in C. elegans
2.2. Q203 Antibiotic Potentially Activates the C. elegans Innate Immune Response and Detoxification Response through Targeting the Electron Transport Chain of Bacteria
2.3. Host Brain Activity Is Required for ΔcyoB E. coli and Q203 to Activate C. elegans Detoxification and Immune Response
2.4. Brain-Mediated Activation of Immune Response by ΔcyoB E. coli and Q203 Enhances the Ability of C. elegans to Resist Pathogenic Invasion
3. Discussion
4. Materials and Methods
4.1. Strains and Maintenance
4.2. Screening for E. coli K-12 Mutants That Activate cyp-14A4 Expression of C. elegans and GO Analysis
4.3. Q203 Supplementation
4.4. Imaging and Analysis of the Expression of Transgenic Reporter cyp-14A4p::GFP
4.5. qPCR Analyses
4.6. RNA Interference in C. elegans
4.7. Quantification of Bacterial Growth
4.8. Generation of Transgenic Worm Strains
4.9. PA14 Slow-Killing Assay
4.10. Macromolecular Docking
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Primers | Sequence |
---|---|---|
rpl-26 | primer F | CTTCGAAGGTCGCTATCACCA |
primer R | TTGACGGTCTCGTCAGTGTG | |
cyp-14A4 | primer F | CGTTTGTAACGCAAGGCGAA |
primer R | CGTTTCCAACGCAAAGCTGA | |
nlp-29 | primer F | TATGGAAGAGGATATGGAGGATATG |
primer R | TCCATGTATTTACTTTCCCCATCC | |
irg-1 | primer F | TGAAACTTGTGGAGGCCTCA |
primer R | TGGCATCTAGTTTCCAGGCT |
Docking Box | x | y | z | |
---|---|---|---|---|
6wti | 101 Å × 101 Å × 101 Å | 161.597 | 168.208 | 157.094 |
CyoB | 62 Å × 70 Å × 60 Å | 170.031 | 161.868 | 151.459 |
CyoA | 106 Å × 64 Å × 56 Å | 160.696 | 182.206 | 141.716 |
CyoC | 88 Å × 64 Å × 56 Å | 160.696 | 170.206 | 180.716 |
CyoD | 81 Å × 56 Å × 65 Å | 160.696 | 180.206 | 180.716 |
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Wu, Y.; Li, G.; Tang, H. Antibiotics Trigger Host Innate Immune Response via Microbiota–Brain Communication in C. elegans. Int. J. Mol. Sci. 2024, 25, 8866. https://doi.org/10.3390/ijms25168866
Wu Y, Li G, Tang H. Antibiotics Trigger Host Innate Immune Response via Microbiota–Brain Communication in C. elegans. International Journal of Molecular Sciences. 2024; 25(16):8866. https://doi.org/10.3390/ijms25168866
Chicago/Turabian StyleWu, Yangyang, Guanqun Li, and Hongyun Tang. 2024. "Antibiotics Trigger Host Innate Immune Response via Microbiota–Brain Communication in C. elegans" International Journal of Molecular Sciences 25, no. 16: 8866. https://doi.org/10.3390/ijms25168866