AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma
Abstract
:1. Introduction
2. Results
2.1. AAV-eNdi1 Provides Benefit in H2O2-Insulted Porcine LC Cells
2.2. AAV-eNdi1 Provides Benefit in Human Glaucomatous LC Cells
2.3. AAV-eNdi1 Provides Benefit in the DBA/2J Murine Model of Glaucoma
3. Discussion
4. Materials and Methods
4.1. Study Design
4.2. Plasmid Constructs and AAV Production
4.3. LC Cell Characterisation, Cell Culture and Mitochondrial Stress Test
4.4. Cellular ROS Assay
4.5. pLC Transfection Immunocytochemistry
4.6. Intravitreal Injection and Electroretinography in Mice
4.7. NADH Oxidation Rate Assay
4.8. Retinal Wholemounts and Immunohistochemistry
4.9. Fluorescence Microscopy
4.10. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Millington-Ward, S.; Palfi, A.; Shortall, C.; Finnegan, L.K.; Bargroff, E.; Post, I.J.M.; Maguire, J.; Irnaten, M.; O′Brien, C.; Kenna, P.F.; et al. AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma. Int. J. Mol. Sci. 2024, 25, 8876. https://doi.org/10.3390/ijms25168876
Millington-Ward S, Palfi A, Shortall C, Finnegan LK, Bargroff E, Post IJM, Maguire J, Irnaten M, O′Brien C, Kenna PF, et al. AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma. International Journal of Molecular Sciences. 2024; 25(16):8876. https://doi.org/10.3390/ijms25168876
Chicago/Turabian StyleMillington-Ward, Sophia, Arpad Palfi, Ciara Shortall, Laura K. Finnegan, Ethan Bargroff, Iris J. M. Post, John Maguire, Mustapha Irnaten, Colm O′Brien, Paul F. Kenna, and et al. 2024. "AAV-NDI1 Therapy Provides Significant Benefit to Murine and Cellular Models of Glaucoma" International Journal of Molecular Sciences 25, no. 16: 8876. https://doi.org/10.3390/ijms25168876