Exosomes from Human iPSC-Derived Retinal Organoids Enhance Corneal Epithelial Wound Healing
Abstract
:1. Introduction
2. Results
2.1. Uptake of Exosomes Derived from ROs by Mouse Corneas
2.2. RO-Derived Exosomes Enhanced Epithelial Wound Healing
2.3. RO-Derived Exosomes Promote Wound Healing by Cell Proliferation and Immunosuppression
2.4. Differential Expression of the Genes in the Corneas Treated with Exo-ROs
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. hiPSC Culture into Three-Dimensional ROs
4.3. Exosome Isolation and NTA
4.4. Exosome Identification: TEM and Western Blotting
4.5. Development of the Corneal Injury Model and Exosome Instillation
4.6. Exosome Uptake Detection
4.7. 5-ethyl-2′-deoxyuridine Assay
4.8. Quantitative Reverse Transcription-Polymerase Chain Reaction
4.9. RNA Sequencing
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Forward | Reverse | Transcription ID |
---|---|---|---|
TNF-α | TCAAACCCTGGTATGAGCCC | ACCCATTCCCTTCACAGAGC | ENSMUST00000025263.15 |
CCL2 | AGGTCCCTGTCATGCTTCTG | TCTGGACCCATTCCTTCTTG | ENSMUST00000000193.6 |
CCL5 | ACTCCCTGCTGCTTTGCCTAC | GAGGTTCCTTCGAGTGACA | ENSMUST00000035938.3 |
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Lee, S.; Han, J.; Yang, J.; Lyu, J.; Park, H.; Bang, J.; Kim, Y.; Chang, H.; Park, T. Exosomes from Human iPSC-Derived Retinal Organoids Enhance Corneal Epithelial Wound Healing. Int. J. Mol. Sci. 2024, 25, 8925. https://doi.org/10.3390/ijms25168925
Lee S, Han J, Yang J, Lyu J, Park H, Bang J, Kim Y, Chang H, Park T. Exosomes from Human iPSC-Derived Retinal Organoids Enhance Corneal Epithelial Wound Healing. International Journal of Molecular Sciences. 2024; 25(16):8925. https://doi.org/10.3390/ijms25168925
Chicago/Turabian StyleLee, Sihyung, Jungwoo Han, Jinyoung Yang, Jungmook Lyu, Hyosong Park, Jihong Bang, Yeji Kim, Hunsoo Chang, and Taekwann Park. 2024. "Exosomes from Human iPSC-Derived Retinal Organoids Enhance Corneal Epithelial Wound Healing" International Journal of Molecular Sciences 25, no. 16: 8925. https://doi.org/10.3390/ijms25168925