The AtMINPP Gene, Encoding a Multiple Inositol Polyphosphate Phosphatase, Coordinates a Novel Crosstalk between Phytic Acid Metabolism and Ethylene Signal Transduction in Leaf Senescence
Abstract
:1. Introduction
2. Results
2.1. Isolation and Identification of the Multiple Inositol Polyphosphate Phosphatase Gene AtMINPP in Arabidopsis
2.2. AtMINPP Involves the Regulation of Leaf Senescence
2.3. AtMINPP Mediates the Ethylene Signaling in Leaf Senescence
2.4. EIN3 Binds to the Promoter of AtMINPP Both In Vitro and In Vivo
2.5. AtMINPP Mediates Ethylene Signaling to Accelerate Leaf Senescence
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Sequence Analysis and Alignment
4.3. Acid Phosphatase Activity, Phytase Activity, and Phytic Acid Content Assay
4.4. Measurement of Chlorophyll Content
4.5. Chlorophyll Fluorescence Measurement of Fv/Fm
4.6. Measurement of Ion Leakage
4.7. Real-Time Quantitative PCR Analysis
4.8. Yeast One-Hybrid Library Screening, Yeast One-Hybrid, and ChIP Assay
4.9. Treatment with ACC, AgNO3, and AVG
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Peng, X.; Li, H.; Xu, W.; Yang, Q.; Li, D.; Fan, T.; Li, B.; Ding, J.; Ku, W.; Deng, D.; et al. The AtMINPP Gene, Encoding a Multiple Inositol Polyphosphate Phosphatase, Coordinates a Novel Crosstalk between Phytic Acid Metabolism and Ethylene Signal Transduction in Leaf Senescence. Int. J. Mol. Sci. 2024, 25, 8969. https://doi.org/10.3390/ijms25168969
Peng X, Li H, Xu W, Yang Q, Li D, Fan T, Li B, Ding J, Ku W, Deng D, et al. The AtMINPP Gene, Encoding a Multiple Inositol Polyphosphate Phosphatase, Coordinates a Novel Crosstalk between Phytic Acid Metabolism and Ethylene Signal Transduction in Leaf Senescence. International Journal of Molecular Sciences. 2024; 25(16):8969. https://doi.org/10.3390/ijms25168969
Chicago/Turabian StylePeng, Xiaoyun, Haiou Li, Wenzhong Xu, Qian Yang, Dongming Li, Tingting Fan, Bin Li, Junhui Ding, Wenzhen Ku, Danyi Deng, and et al. 2024. "The AtMINPP Gene, Encoding a Multiple Inositol Polyphosphate Phosphatase, Coordinates a Novel Crosstalk between Phytic Acid Metabolism and Ethylene Signal Transduction in Leaf Senescence" International Journal of Molecular Sciences 25, no. 16: 8969. https://doi.org/10.3390/ijms25168969