STAT3 Increases CVB3 Replication and Acute Pancreatitis and Myocarditis Pathology via Impeding Nuclear Translocation of STAT1 and Interferon-Stimulated Gene Expression
Abstract
:1. Introduction
2. Results
2.1. CVB3 Upregulates p-STAT3 but Not p-STAT1 Expression in the Pancreases and Hearts of Mice
2.2. CVB3 Infection Increases Nuclear Import of p-STAT3 but Blunts Nuclear Translocation of p-STAT1
2.3. STAT3 Inhibition Rescues Nuclear Translocation of p-STAT1 and Enhances ISG Expression in CVB3-Infected Cell
2.4. STAT3 Inhibitor Restricts CVB3 Replication via STAT1-Dependent Way
2.5. CVB3 VP1 Induces STAT3 Activation, Which Induces Expression of Negative Regulator, SOCS3, to Block pSTAT1/ISG Expression
2.6. Therapeutic STAT3 Inhibitor Treatment Mitigates AP and VMC Pathology via Rescuing ISG Induction
3. Discussion
4. Materials and Methods
4.1. Animals, Virus and Cells
4.2. CVB3-Induced Acute Pancreatitis and Histopathology Evaluation
4.3. Cell Treatment and Virus Infection
4.4. Plaque Assay
4.5. Western Blot
4.6. Enzyme-Linked Immunosorbent Assay (ELISA)
4.7. Nucleic and Cytoplasmic Fraction Purification
4.8. Luciferase Reporter Assay
4.9. shRNA Knockdown
4.10. Quantitative Real-Time PCR (qPCR)
4.11. STAT3 Inhibitor Treatment Experiment
4.12. Immunofluorescent Staining
4.13. Confocal Laser Scanning Microscopy
4.14. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liang, T.; Zhang, Z.; Bai, Z.; Xu, L.; Xu, W. STAT3 Increases CVB3 Replication and Acute Pancreatitis and Myocarditis Pathology via Impeding Nuclear Translocation of STAT1 and Interferon-Stimulated Gene Expression. Int. J. Mol. Sci. 2024, 25, 9007. https://doi.org/10.3390/ijms25169007
Liang T, Zhang Z, Bai Z, Xu L, Xu W. STAT3 Increases CVB3 Replication and Acute Pancreatitis and Myocarditis Pathology via Impeding Nuclear Translocation of STAT1 and Interferon-Stimulated Gene Expression. International Journal of Molecular Sciences. 2024; 25(16):9007. https://doi.org/10.3390/ijms25169007
Chicago/Turabian StyleLiang, Tianming, Zhipeng Zhang, Zhenxin Bai, Le Xu, and Wei Xu. 2024. "STAT3 Increases CVB3 Replication and Acute Pancreatitis and Myocarditis Pathology via Impeding Nuclear Translocation of STAT1 and Interferon-Stimulated Gene Expression" International Journal of Molecular Sciences 25, no. 16: 9007. https://doi.org/10.3390/ijms25169007