Development of Fusion-Based Assay as a Drug Screening Platform for Nipah Virus Utilizing Baculovirus Expression Vector System
Abstract
:1. Introduction
2. Results and Discussion
2.1. NiV Membrane Proteins and EphrinB2 Were Expressed on the Surface of Infected Sf21 Cells
2.2. NiV-Induced Syncytium Formation Was Observed under Low pH- and Cholesterol-Supplemented Culture Condition
2.3. The Compounds That Showed Fusion Inhibition against NiV
2.4. In Silico Results Show That Suramin Binds Effectively to NiV-G’s Central Hole and EphrinB2’s G-H Loop
3. Materials and Methods
3.1. Cell Culture and Compound Preparation
3.2. Preparation of Donor Plasmid and Generation of Recombinant Baculoviruses
3.3. Determination of Target Protein Expression in Infected Insect Cells
3.4. Syncytium Inhibition Assay
3.5. In Silico Analysis of the Binding of Suramin and Oleanolic Acid on NiV-G and EphrinB2
3.6. Contact Probability Distribution as a Tool for Determining Drug Contact Profile
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Binding Energy (kcal/mol) | ||
---|---|---|
Suramin | Oleanolic Acid (OA) | |
NiV-G | −49.57 ± 1.00 | −17.52 ± 0.48 |
EphrinB2 | −32.69 ± 0.69 | −27.21 ± 0.66 |
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Sari, I.P.; Ortiz, C.L.D.; Yang, L.-W.; Chen, M.-H.; Perng, M.-D.; Wu, T.-Y. Development of Fusion-Based Assay as a Drug Screening Platform for Nipah Virus Utilizing Baculovirus Expression Vector System. Int. J. Mol. Sci. 2024, 25, 9102. https://doi.org/10.3390/ijms25169102
Sari IP, Ortiz CLD, Yang L-W, Chen M-H, Perng M-D, Wu T-Y. Development of Fusion-Based Assay as a Drug Screening Platform for Nipah Virus Utilizing Baculovirus Expression Vector System. International Journal of Molecular Sciences. 2024; 25(16):9102. https://doi.org/10.3390/ijms25169102
Chicago/Turabian StyleSari, Indah Permata, Christopher Llynard D. Ortiz, Lee-Wei Yang, Ming-Hsiang Chen, Ming-Der Perng, and Tzong-Yuan Wu. 2024. "Development of Fusion-Based Assay as a Drug Screening Platform for Nipah Virus Utilizing Baculovirus Expression Vector System" International Journal of Molecular Sciences 25, no. 16: 9102. https://doi.org/10.3390/ijms25169102