Revisiting the Injury Mechanism of Goat Sperm Caused by the Cryopreservation Process from a Perspective of Sperm Metabolite Profiles
Abstract
:1. Introduction
2. Results
2.1. Metabolic Findings of Sperm Samples Using UHPLC-QTOF-MS
2.2. Identification and Functional Annotation of Differential Metabolites
2.3. Classification and Hierarchical Clustering of Significantly DAMs
3. Discussion
4. Materials and Methods
4.1. Study Design and Sample Collection
4.2. Cooling and Freeze-Thaw Processing
4.3. Extraction of Metabolite
4.4. UHPLC-QTOF-MS
4.5. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
DAMs | Differentially Abundant Metabolites |
TCA | tricarboxylic acid |
ATP | adenosine triphosphate |
cAMP-PKA | cyclic adenosine monophosphate-dependent protein kinase |
QC | quality control |
UHPLC-QTOF-MS | ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry |
POS | positive mode |
PCA | principal component analysis |
OPLS-DA | orthogonal Partial least square discriminant |
ESI | electrospray ionization |
ROS | reactive oxygen species |
acetyl-CoA | acetyl coenzyme A |
NADH | nicotinamide adenine dinucleotide |
MUFAs | monounsaturated fatty acids |
PUFAs | polyunsaturated fatty acids |
LA | linoleic acid |
AA | arachidonic acid |
DHA | docosahexaenoic acid |
PLA2 | phospholipase A2 |
L-Cth | L-cystathionine |
GSH-Px | glutathione peroxidase |
SOD | superoxide dismutase |
CAT | catalase |
MDA | malondialdehyde |
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Li, C.; Lv, C.; Larbi, A.; Liang, J.; Yang, Q.; Wu, G.; Quan, G. Revisiting the Injury Mechanism of Goat Sperm Caused by the Cryopreservation Process from a Perspective of Sperm Metabolite Profiles. Int. J. Mol. Sci. 2024, 25, 9112. https://doi.org/10.3390/ijms25169112
Li C, Lv C, Larbi A, Liang J, Yang Q, Wu G, Quan G. Revisiting the Injury Mechanism of Goat Sperm Caused by the Cryopreservation Process from a Perspective of Sperm Metabolite Profiles. International Journal of Molecular Sciences. 2024; 25(16):9112. https://doi.org/10.3390/ijms25169112
Chicago/Turabian StyleLi, Chunyan, Chunrong Lv, Allai Larbi, Jiachong Liang, Qige Yang, Guoquan Wu, and Guobo Quan. 2024. "Revisiting the Injury Mechanism of Goat Sperm Caused by the Cryopreservation Process from a Perspective of Sperm Metabolite Profiles" International Journal of Molecular Sciences 25, no. 16: 9112. https://doi.org/10.3390/ijms25169112