Synergistic Toxicity of Pollutant and Ultraviolet Exposure from a Mitochondrial Perspective
Abstract
:1. Introduction
2. Results
2.1. Affinity of BaP for Mitochondria
2.2. Lipid Peroxidation Is Induced in Isolated Mitochondria and Cardiolipin-Containing Multilamellar Vesicles (MLVs) Exposed to BaP/UVAssl
2.3. Co-Exposure to BaP/UVAssl Destabilizes Mitochondrial Inner Membranes and Leads to Mitochondrial Fission
2.4. Mitochondrial Membrane Potential and Metabolism Is Depleted by BaP/UVAssl
3. Discussion
4. Material and Methods
4.1. Cell Culture
4.2. Mitochondrial Extraction
4.3. BaP Affinity to Mitochondria
4.4. Pollutants and UVA Exposure
4.5. Antioxidant Treatments
4.6. MDA Evaluation
4.7. Preparation of MLVs
4.8. Transmission Electron Microscopy (TEM)
4.9. Mitochondrial Fission
4.10. Membrane Potential Assessment
4.11. Metabolism Assessment
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Larnac, E.; Méthot, S.; Pelchat, F.; Millette, M.-A.; Montoni, A.; Salesse, C.; Haydont, V.; Marrot, L.; Rochette, P.J. Synergistic Toxicity of Pollutant and Ultraviolet Exposure from a Mitochondrial Perspective. Int. J. Mol. Sci. 2024, 25, 9146. https://doi.org/10.3390/ijms25179146
Larnac E, Méthot S, Pelchat F, Millette M-A, Montoni A, Salesse C, Haydont V, Marrot L, Rochette PJ. Synergistic Toxicity of Pollutant and Ultraviolet Exposure from a Mitochondrial Perspective. International Journal of Molecular Sciences. 2024; 25(17):9146. https://doi.org/10.3390/ijms25179146
Chicago/Turabian StyleLarnac, Eloïse, Sébastien Méthot, Frédéric Pelchat, Marc-Antoine Millette, Alicia Montoni, Christian Salesse, Valérie Haydont, Laurent Marrot, and Patrick J. Rochette. 2024. "Synergistic Toxicity of Pollutant and Ultraviolet Exposure from a Mitochondrial Perspective" International Journal of Molecular Sciences 25, no. 17: 9146. https://doi.org/10.3390/ijms25179146