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Axonal Growth and Fasciculation of Spinal Neurons Promoted by Aldynoglia in Alkaline Fibrin Hydrogel: Influence of Tol-51 Sulfoglycolipid
1
Hospital Nacional de Parapléjicos, Servicio de Salud de Castilla La Mancha, 45071 Toledo, Spain
2
Departamento de Química Bio-Orgánica, Instituto de Química Orgánica General (IQOG-CSIC), CSIC, 28006 Madrid, Spain
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Int. J. Mol. Sci. 2024, 25(17), 9173; https://doi.org/10.3390/ijms25179173 (registering DOI)
Submission received: 2 July 2024
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Revised: 13 August 2024
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Accepted: 20 August 2024
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Published: 23 August 2024
Abstract
Traumatic spinal cord injury (tSCI) has complex pathophysiological events that begin after the initial trauma. One such event is fibroglial scar formation by fibroblasts and reactive astrocytes. A strong inhibition of axonal growth is caused by the activated astroglial cells as a component of fibroglial scarring through the production of inhibitory molecules, such as chondroitin sulfate proteoglycans or myelin-associated proteins. Here, we used neural precursor cells (aldynoglia) as promoters of axonal growth and a fibrin hydrogel gelled under alkaline conditions to support and guide neuronal cell growth, respectively. We added Tol-51 sulfoglycolipid as a synthetic inhibitor of astrocyte and microglia in order to test its effect on the axonal growth-promoting function of aldynoglia precursor cells. We obtained an increase in GFAP expression corresponding to the expected glial phenotype for aldynoglia cells cultured in alkaline fibrin. In co-cultures of dorsal root ganglia (DRG) and aldynoglia, the axonal growth promotion of DRG neurons by aldynoglia was not affected. We observed that the neural precursor cells first clustered together and then formed niches from which aldynoglia cells grew and connected to groups of adjacent cells. We conclude that the combination of alkaline fibrin with synthetic sulfoglycolipid Tol-51 increased cell adhesion, cell migration, fasciculation, and axonal growth capacity, promoted by aldynoglia cells. There was no negative effect on the behavior of aldynoglia cells after the addition of sulfoglycolipid Tol-51, suggesting that a combination of aldynoglia plus alkaline fibrin and Tol-51 compound could be useful as a therapeutic strategy for tSCI repair.
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MDPI and ACS Style
Buzoianu-Anguiano, V.; Arriero-Cabañero, A.; Fernández-Mayoralas, A.; Torres-Llacsa, M.; Doncel-Pérez, E.
Axonal Growth and Fasciculation of Spinal Neurons Promoted by Aldynoglia in Alkaline Fibrin Hydrogel: Influence of Tol-51 Sulfoglycolipid. Int. J. Mol. Sci. 2024, 25, 9173.
https://doi.org/10.3390/ijms25179173
AMA Style
Buzoianu-Anguiano V, Arriero-Cabañero A, Fernández-Mayoralas A, Torres-Llacsa M, Doncel-Pérez E.
Axonal Growth and Fasciculation of Spinal Neurons Promoted by Aldynoglia in Alkaline Fibrin Hydrogel: Influence of Tol-51 Sulfoglycolipid. International Journal of Molecular Sciences. 2024; 25(17):9173.
https://doi.org/10.3390/ijms25179173
Chicago/Turabian Style
Buzoianu-Anguiano, Vinnitsa, Alejandro Arriero-Cabañero, Alfonso Fernández-Mayoralas, Mabel Torres-Llacsa, and Ernesto Doncel-Pérez.
2024. "Axonal Growth and Fasciculation of Spinal Neurons Promoted by Aldynoglia in Alkaline Fibrin Hydrogel: Influence of Tol-51 Sulfoglycolipid" International Journal of Molecular Sciences 25, no. 17: 9173.
https://doi.org/10.3390/ijms25179173
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