Systematic Analysis of the BrHAT Gene Family and Physiological Characteristics of Brassica rapa L. Treated with Histone Acetylase and Deacetylase Inhibitors under Low Temperature
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification and Phylogenetic Analysis of BrHAT Genes
2.2. Conserved Domains, Structure, and Replicate Sequences of BrHAT Genes
2.3. Analysis of Covariance within and between Species in BrHAT Genes
2.4. Expression Analysis of BrHATs under Abiotic Stress
2.5. Gene Ontology (GO) Enrichment Analysis of BrHAT Genes
2.6. Protein Interaction Prediction of BrHAT Genes
2.7. Effects of Different Inhibitor Treatments on HAT and HDAC Activities in B. rapa L.
2.8. Effects of Inhibitor Treatments on the Seed Germination and Growth of Different Cold-Resistant Varieties
2.9. Effect of Exogenous Inhibitors on Antioxidant Enzyme Activities in B. rapa L.
2.10. Effect of Exogenous Curcumin Application on the Soluble Matter Content of B. rapa L.
2.11. Expression Analysis of BrHATs after Inhibitor Treatment
3. Discussion
3.1. Analysis of HAT Genes in B. rapa L.
3.2. Relationships between HAT and HDAC Activities and Cold Tolerance
3.3. Effects of TSA Treatment on the Growth, Development, and Physiological Indexes of B. rapa L.
3.4. Effects of Curcumin Treatment on the Growth, Development, and Physiological Indexes of B. rapa L.
3.5. The Role of BrHATs in Growth, Development, and the Response to Low-Temperature Stress
4. Materials and Methods
4.1. HAT Gene Identification and Analysis of Phylogenetic Relationships, Chromosomal Location, Conserved Domains, and Gene Structure
4.2. Plant Materials, Growth Conditions, and Treatments
- TSA treatment
- B.
- Curcumin treatment
4.3. Analysis of Biochemical Indexes
4.4. Expression Pattern Analysis of BrHATs
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Sequence ID | Number of Amino Acid | Molecular Weight/kDa | pI | Instability Index | Hydrophilia | Aliphatic Index |
---|---|---|---|---|---|---|---|
BrHAC1 | Brapa01T000131.1 | 374 | 42,686.6 | 9.08 | 55.25 | 84.41 | −0.336 |
BrTAF1 | Brapa01T003430.1 | 1772 | 201,196.61 | 5.69 | 54.09 | 70.56 | −0.799 |
BrTAF2 | Brapa01T003498.1 | 305 | 34,158.23 | 6.94 | 28.92 | 102.26 | −0.429 |
BrHAC9 | Brapa01T003886.1 | 1637 | 184,450.74 | 8.85 | 50.11 | 66.76 | −0.671 |
BrHAC10 | Brapa02T001822.1 | 1012 | 116,033 | 8.01 | 44.88 | 70.3 | −0.565 |
BrHAC11 | Brapa02T002581.1 | 1653 | 185,472.72 | 8.71 | 57.08 | 62.03 | −0.737 |
BrHAC2 | Brapa02T004562.1 | 368 | 41,825.9 | 9.45 | 64.14 | 87.39 | −0.313 |
BrTAF3 | Brapa03T002862.1 | 305 | 34,192.25 | 6.94 | 29.2 | 100.98 | −0.434 |
BrHAC12 | Brapa03T003605.1 | 1603 | 181,371.88 | 8.81 | 52.63 | 65.31 | −0.679 |
BrHAC3 | Brapa03T006148.1 | 379 | 43,316.25 | 8.92 | 54.44 | 83.83 | −0.405 |
BrHAG1 | Brapa04T000545.1 | 525 | 59,015.22 | 6.09 | 41.82 | 70.1 | −0.62 |
BrHAG2 | Brapa04T000549.1 | 548 | 60,926.09 | 6.13 | 40.5 | 69.29 | −0.624 |
BrTAF4 | Brapa05T002439.1 | 229 | 25,685.49 | 6.86 | 29.17 | 100.87 | −0.431 |
BrHAC8 | Brapa06T001216.1 | 990 | 110,352.88 | 6.71 | 51.87 | 69.22 | −0.598 |
BrHAM1 | Brapa06T002435.1 | 160 | 18,855.76 | 6.17 | 48.73 | 79.25 | −0.456 |
BrTAF5 | Brapa06T002581.1 | 381 | 42,699.02 | 7 | 29.22 | 101.05 | −0.436 |
BrHAC4 | Brapa06T002586.1 | 380 | 43,636.84 | 9.16 | 44.78 | 82.84 | −0.357 |
BrHAC13 | Brapa07T003346.1 | 876 | 100,268.81 | 6.36 | 57.76 | 75.21 | −0.509 |
BrTAF6 | Brapa08T000884.1 | 1893 | 213,267.71 | 5.35 | 52.28 | 72.09 | −0.816 |
BrTAF7 | Brapa08T001640.1 | 229 | 25,685.49 | 6.86 | 29.17 | 100.87 | −0.431 |
BrHAC14 | Brapa08T003011.1 | 1578 | 176,696.25 | 8.74 | 53.39 | 64.27 | −0.724 |
BrHAC5 | Brapa08T003012.1 | 297 | 32,073.09 | 6.76 | 57.55 | 75.52 | −0.525 |
BrHAC6 | Brapa08T003480.1 | 369 | 41,612.29 | 9.3 | 52.74 | 81.36 | −0.341 |
BrHAC15 | Brapa09T000822.1 | 1653 | 183,826.65 | 8.72 | 50.79 | 65.36 | −0.667 |
BrTAF8 | Brapa09T004162.1 | 229 | 25,685.49 | 6.86 | 29.17 | 100.87 | −0.431 |
BrHAM2 | Brapa09T005768.1 | 465 | 54,007.8 | 6.81 | 44.2 | 83.2 | −0.515 |
BrHAM3 | Brapa10T000566.1 | 441 | 50,864.11 | 7.56 | 40.61 | 78.25 | −0.619 |
BrHAC15 | Brapa10T001880.1 | 1712 | 192,652.85 | 5.58 | 42.45 | 90.95 | −0.21 |
BrHAC7 | Brapa10T002727.1 | 955 | 106,960.73 | 8.11 | 46.98 | 89.75 | −0.155 |
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Bian, L.; Fahim, A.M.; Wu, J.; Liu, L.; Pu, Y.; Ma, L.; Fang, Y.; Zhang, D.; Yang, G.; Wang, W.; et al. Systematic Analysis of the BrHAT Gene Family and Physiological Characteristics of Brassica rapa L. Treated with Histone Acetylase and Deacetylase Inhibitors under Low Temperature. Int. J. Mol. Sci. 2024, 25, 9200. https://doi.org/10.3390/ijms25179200
Bian L, Fahim AM, Wu J, Liu L, Pu Y, Ma L, Fang Y, Zhang D, Yang G, Wang W, et al. Systematic Analysis of the BrHAT Gene Family and Physiological Characteristics of Brassica rapa L. Treated with Histone Acetylase and Deacetylase Inhibitors under Low Temperature. International Journal of Molecular Sciences. 2024; 25(17):9200. https://doi.org/10.3390/ijms25179200
Chicago/Turabian StyleBian, Liang, Abbas Muhammad Fahim, Junyan Wu, Lijun Liu, Yuanyuan Pu, Li Ma, Yan Fang, Dan Zhang, Gang Yang, Wangtian Wang, and et al. 2024. "Systematic Analysis of the BrHAT Gene Family and Physiological Characteristics of Brassica rapa L. Treated with Histone Acetylase and Deacetylase Inhibitors under Low Temperature" International Journal of Molecular Sciences 25, no. 17: 9200. https://doi.org/10.3390/ijms25179200