A PLGA/Silk Fibroin Nanofibre Membrane Loaded with Natural Flavonoid Compounds Extracted from Green Cocoons for Wound Healing
Abstract
:1. Introduction
2. Results and Discussion
2.1. Characterization of PLGA/SF Nanofibre Membranes
2.2. Fabrication and Characterization of FL-Loaded P-SF Nanofibre Membranes
2.3. Release of FLs from FL-Loaded P-SF Membranes
2.4. Anti-Oxidation and Antibacterial Properties of the P-SF-nFL Nanofibre Membranes
2.5. Biocompatibility of the P-SF-nFLs Nanofibre Membranes
2.6. Anti-Inflammatory Analysis of P-SF-nFLs Nanofibre Membranes
2.7. In Vivo Wound Healing and Histological Analysis
2.8. Effect of FLs on Expression of Genes Involved in Wound Healing
3. Materials and Methods
3.1. Materials
3.2. Extraction of Flavonoids and Preparation of Regenerated Silk Fibroin
3.3. Fabrication of PLGA/SF-nFL Nanofibre Membranes
3.4. Characterization of Composite Nanofibre Membranes
3.5. In Vitro Release of FLs from FL-Loaded Membranes
3.6. Antioxidant and Antimicrobial Activity of the Nanofibre Membranes
3.7. In Vitro Biocompatibility of PLGA/SF-nFL Membranes
3.8. Anti-Inflammatory Testing of PLGA/SF-nFL Membranes
3.9. In Vivo Wound Healing with Nanofibre Membranes
3.10. Histological Analysis
3.11. Real-Time qPCR Examination
3.12. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Sample Name | Concentration of PLGA (wt%) | Concentration of SF (wt%) | Concentration of FLs (mg/g to Dry Matter) |
---|---|---|---|
SF | - | 10 | - |
50PLGA/50SF | 5 | 5 | - |
60PLGA/40SF | 6 | 4 | - |
80PLGA/20SF | 8 | 2 | - |
PLGA | 10 | - | - |
80PLGA/20SF/nFLs | 8 | 2 | 3, 6, 15, 30 |
Gene | Forward Primer | Reverse Primer |
---|---|---|
GAPDH | CCTTCCGTGTTCCTACCCC | ACCAGGAAATGAGCTTGACA |
IL-1β | AACAAACCCTGCAGTGGT TCG | AGCTGCTTCAGACACTTGCAC |
IL-10 | CCATCATGCCTGGCT CAG CAC | TGTACTGGCCCCTGCTGATCC |
TNF-α | GAAGCTCCCTCAGCGAGGACA | TTGGGCCAGTGAGTGAAAGGG |
VEGF | GAGACCCTGGTGGACATCTT | GATCCGCATGATCTGCATAGG |
TGF-β | AACAATTCCTGGCGTTACCTT | TGTATTCCGTCTCCTTGGTTC |
EGF | GCCACGGTTACATTCACTCC | CCAAATCGCCTTCTCTTTCA |
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Chen, X.; Liu, J.; Lu, Y.; Liu, H.; Cheng, L.; Li, Z.; Dai, F. A PLGA/Silk Fibroin Nanofibre Membrane Loaded with Natural Flavonoid Compounds Extracted from Green Cocoons for Wound Healing. Int. J. Mol. Sci. 2024, 25, 9263. https://doi.org/10.3390/ijms25179263
Chen X, Liu J, Lu Y, Liu H, Cheng L, Li Z, Dai F. A PLGA/Silk Fibroin Nanofibre Membrane Loaded with Natural Flavonoid Compounds Extracted from Green Cocoons for Wound Healing. International Journal of Molecular Sciences. 2024; 25(17):9263. https://doi.org/10.3390/ijms25179263
Chicago/Turabian StyleChen, Xiang, Jiaqi Liu, Yaru Lu, Huijun Liu, Lan Cheng, Zhi Li, and Fangyin Dai. 2024. "A PLGA/Silk Fibroin Nanofibre Membrane Loaded with Natural Flavonoid Compounds Extracted from Green Cocoons for Wound Healing" International Journal of Molecular Sciences 25, no. 17: 9263. https://doi.org/10.3390/ijms25179263