Genome-Wide Identification of NAC Gene Family Members of Tree Peony (Paeonia suffruticosa Andrews) and Their Expression under Heat and Waterlogging Stress
Abstract
:1. Introduction
2. Results
2.1. Identification of PsNAC Family Members and Analysis of Physicochemical Properties
2.2. Phylogenetic Analysis and Classification of PsNAC
2.3. Protein Conservation Motif and Gene Structure Analysis of PsNAC Family
2.4. Analysis of Cis-Acting Elements in the Promoter Region of PsNAC Genes
2.5. Expression Pattern Analysis of PsNAC Genes
2.6. Annotation and Enrichment Analysis of PsNAC Genes
2.7. qRT-PCR Validation of Differentially Expressed PsNAC Genes
3. Discussion
4. Materials and Methods
4.1. Identification and Testing of Transcription Factors
4.2. Analysis of Physicochemical Properties of Protein Sequences
4.3. Construction of Phylogenetic Tree
4.4. Conserved Sequence Comparison and Motif Analysis
4.5. Analysis of Cis-Acting Elements in the Promoter Region
4.6. Gene Expression Analysis
4.7. Annotation and Enrichment Analysis in GO and KEGG Databases
4.8. Plant Material and Stress Treatments
4.9. qRT-PCR Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Gene Name | Expression Level | log2 (Fold Change) | q-Value |
---|---|---|---|---|
Heat stress | PsNAC06 | up | 3.80155351 | 0.000157825 |
PsNAC23 | down | −1.142697267 | 3.12 × 10−9 | |
PsNAC38 | down | −6.598682031 | 0.0000937 | |
PsNAC41 | down | −2.098132113 | 5.41 × 10−9 | |
PsNAC47 | down | −2.611193255 | 0.0000686 | |
Waterlogging stress | PsNAC37 | up | 4.289772524 | 3.41 × 10−5 |
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Wang, Q.; Zhou, L.; Yuan, M.; Peng, F.; Zhu, X.; Wang, Y. Genome-Wide Identification of NAC Gene Family Members of Tree Peony (Paeonia suffruticosa Andrews) and Their Expression under Heat and Waterlogging Stress. Int. J. Mol. Sci. 2024, 25, 9312. https://doi.org/10.3390/ijms25179312
Wang Q, Zhou L, Yuan M, Peng F, Zhu X, Wang Y. Genome-Wide Identification of NAC Gene Family Members of Tree Peony (Paeonia suffruticosa Andrews) and Their Expression under Heat and Waterlogging Stress. International Journal of Molecular Sciences. 2024; 25(17):9312. https://doi.org/10.3390/ijms25179312
Chicago/Turabian StyleWang, Qun, Lin Zhou, Meng Yuan, Fucheng Peng, Xiangtao Zhu, and Yan Wang. 2024. "Genome-Wide Identification of NAC Gene Family Members of Tree Peony (Paeonia suffruticosa Andrews) and Their Expression under Heat and Waterlogging Stress" International Journal of Molecular Sciences 25, no. 17: 9312. https://doi.org/10.3390/ijms25179312