Genome Mining and Biological Engineering of Type III Borosins from Bacteria
Abstract
:1. Introduction
2. Results
2.1. Genome Mining of New Type III Borosins
2.2. Validation of the New Type III Borosin KchMA Derived from Bacteria
2.3. Introduction of Artificial Protease Sit to Release N-Methylation Peptide
2.4. Rational Evolution of Methyltransferase Domain of KchMATEV
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Genome Mining
4.3. Construction of Phylogenetic Tree
4.4. AI-Assisted Protein Engineering
4.5. Construction of Mutants
4.6. Protein Expression
4.7. Protein Purification
4.8. The Enzymatic Hydrolysis of Borosins
4.9. LC-MS/MS Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Mutants Name | Position 49 | Position 188 |
---|---|---|
KchMATEV | C | F |
M1 | V | Y |
M2 | T | F |
M3 | A | W |
M4 | M | M |
M5 | S | V |
M6 | C | L |
M7 | L | H |
M8 | G | T |
M9 | I | I |
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Xu, K.; Guo, S.; Zhang, W.; Deng, Z.; Zhang, Q.; Ding, W. Genome Mining and Biological Engineering of Type III Borosins from Bacteria. Int. J. Mol. Sci. 2024, 25, 9350. https://doi.org/10.3390/ijms25179350
Xu K, Guo S, Zhang W, Deng Z, Zhang Q, Ding W. Genome Mining and Biological Engineering of Type III Borosins from Bacteria. International Journal of Molecular Sciences. 2024; 25(17):9350. https://doi.org/10.3390/ijms25179350
Chicago/Turabian StyleXu, Kuang, Sijia Guo, Wei Zhang, Zixin Deng, Qi Zhang, and Wei Ding. 2024. "Genome Mining and Biological Engineering of Type III Borosins from Bacteria" International Journal of Molecular Sciences 25, no. 17: 9350. https://doi.org/10.3390/ijms25179350