Kinetics of Riboflavin Production by Hyphopichia wangnamkhiaoensis under Varying Nutritional Conditions
Abstract
:1. Introduction
2. Results and Discussion
2.1. Effect of Various Carbon Sources on Riboflavin Production by H. wangnamkhiaoensis in a Bubble Column Bioreactor
2.2. Effect of Various Nitrogen Sources on Riboflavin Production by H. wangnamkhiaoensis
2.2.1. Flask Screening of Nitrogen Sources for Riboflavin Production by H. wangnamkhiaoensis
2.2.2. Effect of the Selected Nitrogen Sources on Cell Growth, Glucose Consumption, and Riboflavin Production by H. wangnamkhiaoensis in a Bubble Column Bioreactor
2.3. Effect of Histidine, Methionine, and Tryptophan on Cell Growth, Glucose Consumption, and Riboflavin Production by H. wangnamkhiaoensis in a Bubble Column Bioreactor
2.4. Effect of Various Vitamins on Cell Growth, Glucose Consumption, and Riboflavin Production by H. wangnamkhiaoensis in a Bubble Column Bioreactor
2.5. Formulation of a Culture Medium for Riboflavin Production by H. wangnamkhiaoensis in a Bubble Column Bioreactor
3. Materials and Methods
3.1. Micro-Organism
3.2. Culture Media
3.3. Pneumatic Bubble Column Bioreactor
3.4. Kinetic Study of the Effect of Different Nutritional Factors on Riboflavin Production by H. wangnamkhiaoensis
3.4.1. Effect of Different Carbon Sources
3.4.2. Influence of Different Nitrogen Sources
3.4.3. Effect of Some Amino Acids
3.4.4. Influence of Some Vitamins
3.5. Formulation of a Culture Medium for Riboflavin Production by H. wangnamkhiaoensis
3.6. Analytical Methods
3.6.1. Cell Concentration
3.6.2. Substrate Concentration
3.6.3. Riboflavin Concentration
3.7. Data and Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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ΔXmax (g/L) | μmax (1/h) | Rxmax (g/L·h) | E (%) | P (mg/L) | Rpmax (mg/L·h) | |
---|---|---|---|---|---|---|
Positive control (YNB) | 2.91 ± 0.21 | 0.16 ± 0.02 | 0.15 ± 0.01 | 99.29 ± 0.19 | 8.88 ± 0.42 | 0.37 ± 0.08 |
Negative control (YNB-VF) | 2.15 ± 0.23 | 0.12 ± 0.03 | 0.07 ± 0.01 | 99.55 ± 0.10 | 2.28 ± 0.16 | 0.07 ± 0.01 |
Biotin | 2.61 ± 0.21 | 0.17 ± 0.01 | 0.13 ± 0.01 | 98.92 ± 0.10 | 9.62 ± 0.49 | 0.36 ± 0.001 |
Calcium pantothenate | 2.80 ± 0.35 | 0.14 ± 0.01 | 0.14 ± 0.01 | 99.51 ± 0.01 | 0.91 ± 0.08 | 0.03 ± 0.001 |
Folic acid | 2.86 ± 0.25 | 0.17 ± 0.02 | 0.14 ± 0.01 | 98.40 ± 0.09 | 2.70 ± 0.33 | 0.11 ± 0.01 |
Inositol | 3.24 ± 0.20 | 0.16 ± 0.01 | 0.12 ± 0.01 | 99.65 ± 0.01 | 1.93 ± 0.08 | 0.05 ± 0.001 |
Niacin | 2.24 ± 0.26 | 0.09 ± 0.01 | 0.10 ± 0.03 | 99.89 ± 0.01 | 3.62 ± 0.36 | 0.10 ± 0.02 |
p-Aminobenzoic acid | 2.63 ± 0.26 | 0.17 ± 0.01 | 0.14 ± 0.01 | 98.48 ± 0.01 | 5.32 ± 0.24 | 0.19 ± 0.01 |
Pyridoxine hydrochloride | 2.18 ± 0.08 | 0.05 ± 0.01 | 0.05 ± 0.01 | 99.74 ± 0.01 | 0.65 ± 0.16 | 0.003 ± 0.01 |
Thiamine hydrochloride | 2.48 ± 0.35 | 0.17 ± 0.06 | 0.09 ± 0.01 | 99.74 ± 0.02 | 2.75 ± 0.38 | 0.10 ± 0.01 |
Component | YNB | YNB-w/o-N | YNB-VF | |
---|---|---|---|---|
Amount per Liter | ||||
Carbon source | Glucose | 0 g | 0 g | 10 g |
Nitrogen source | Ammonium sulfate | 5 g | 0 g | 5 g |
Amino acids | L-histidine monohydrocloride | 10 mg | 0 mg | 10 mg |
DL-methionine | 20 mg | 0 mg | 20 mg | |
DL-tryptophan | 20 mg | 0 mg | 20 mg | |
Vitamins | Biotin | 20 μg | 20 μg | 0 μg |
Calcium pantothenate | 2000 μg | 2000 μg | 0 μg | |
Folic acid | 2 μg | 2 μg | 0 μg | |
Inositol | 10,000 μg | 10,000 μg | 0 μg | |
Niacin | 400 μg | 400 μg | 0 μg | |
p-Aminobenzoic acid | 200 μg | 200 μg | 0 μg | |
Pyridoxine hydrochloride | 400 μg | 400 μg | 0 μg | |
Riboflavin | 200 μg | 200 μg | 0 μg | |
Thiamine hydrochloride | 400 μg | 400 μg | 0 μg | |
Trace elements | Boric acid | 500 μg | 500 μg | 500 μg |
Copper sulfate | 40 μg | 40 μg | 40 μg | |
Potassium iodide | 100 μg | 100 μg | 100 μg | |
Manganese sulfate | 400 μg | 400 μg | 400 μg | |
Ferric chloride | 400 μg | 400 μg | 400 μg | |
Sodium molybdate | 200 μg | 200 μg | 200 μg | |
Zinc sulfate | 400 μg | 400 μg | 400 μg | |
Salts | Monobasic potassium phosphate | 0.85 g | 0.85 g | 0.85 g |
Dibasic potassium phosphate | 0.15 g | 0.15 g | 0.15 g | |
Magnesium sulfate | 0.5 g | 0.5 g | 0.5 g | |
Sodium chloride | 0.1 g | 0.1 g | 0.1 g | |
Calcium chloride | 0.1 g | 0.1 g | 0.1 g |
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Jiménez-Nava, R.A.; Chávez-Camarillo, G.M.; Cristiani-Urbina, E. Kinetics of Riboflavin Production by Hyphopichia wangnamkhiaoensis under Varying Nutritional Conditions. Int. J. Mol. Sci. 2024, 25, 9430. https://doi.org/10.3390/ijms25179430
Jiménez-Nava RA, Chávez-Camarillo GM, Cristiani-Urbina E. Kinetics of Riboflavin Production by Hyphopichia wangnamkhiaoensis under Varying Nutritional Conditions. International Journal of Molecular Sciences. 2024; 25(17):9430. https://doi.org/10.3390/ijms25179430
Chicago/Turabian StyleJiménez-Nava, Raziel Arturo, Griselda Ma. Chávez-Camarillo, and Eliseo Cristiani-Urbina. 2024. "Kinetics of Riboflavin Production by Hyphopichia wangnamkhiaoensis under Varying Nutritional Conditions" International Journal of Molecular Sciences 25, no. 17: 9430. https://doi.org/10.3390/ijms25179430