Effect of High Glucose on Embryological Development of Zebrafish, Brachyodanio, Rerio through Wnt Pathway
Abstract
:1. Introduction
2. Results
2.1. Effect of Glucose on Zebrafish Embryo Development
2.2. RNA-Seq Analysis Displayed Significant Gene Dysregulation in the High-Glucose-Treated Embryos
2.3. Functional Analysis of DEGs Using Gene Ontology (GO)Analysis
2.4. KEGG Pathway Analysis
3. Discussion
3.1. Limitations in This Study
3.2. Implications and Future Directions
3.3. Conclusions
4. Materials and Methods
4.1. Materials
4.2. Animal Husbandry
4.2.1. Preparation of D-Glucose Application
4.2.2. Addition of D-Glucose and Collection of Embryos
4.2.3. Hatch Rate Calculation
4.3. Total RNA Extraction
4.4. RNA Seq
4.5. Reads Mapping to the Reference Genome
4.6. Novel Transcript Prediction
4.7. Quantification of Gene Expression Level
4.8. Differential Expression Analysis
4.9. GO and KEGG Enrichment Analysis of Differentially Expressed Genes
4.10. Gene Set Enrichment Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Biological Process | Molecular Function |
---|---|---|
Kcnj1a.2 | Transport; ion transport | Enable ATP-activated inward rectifier potassium channel activity |
Eomesa | Enables DNA binding activity and transcription factor activity in blastoderm, blastodisc, brain, eye, and immune system | DNA-binding and DNA-binding transcription factor activity |
zgc:77439 | Enables N,N-dimethylaniline monooxygenase activity, NADP binding activity and flavin adenine dinucleotide binding activity | NADP binding activity in response to xenobiotic stimulus in endoplasmic reticulum |
foxg1d | Transcription regulation, DNA binding activity | Enables sequence specific double-stranded DNA binding activity in nucleus |
zgc:15840 | Transport; urea transmembrane transporter activity | Enables serine-type endopeptidase activity and urea transmembrane transporter activity |
tbr1b | Commitment of neuronal cells to specific neuron type in forebrain, neuron projection development, transcription regulation | DNA-binding transcription factor activity |
pcdh1g32 | Calcium ion binding activity and cell adhesion | Homophilic cell adhesion via plasma membrane adhesion molecules in the brain |
tert | Telomerase activity; erythrocyte maturation in brain, eye, hear, liver, pleuroperitoneal region, intestinal epithelial structure maintenance | Telomere maintenance via telomerase |
plp1a | myelination | Structural constituent of myelin sheath |
TIAM2 | GTPase activity, axonogenesis regulation, signal transduction | enables GTPase activator activity and guanyl-nucleotide exchange factor activity |
Gene | Biological Process | Molecular Function |
---|---|---|
apoda.2 | lipid metabolic process, response to reactive oxygen species, aging, lipid transport | Enables lipid binding activity |
lct | Carbohydrate metabolic processes, hydrolase activity | Hydrolyzes O-glycosyl compounds |
tnfrsf11b | Response to mechanical stimulus | Tumor necrosis factor receptor; head and scale |
opn5 | Absorption of visible light | Enables 11-cis retinal binding activity and all-trans retinal binding activity |
rgra | G protein-coupled receptor signaling, cellular response to light stimulus and phototransduction | Enables G protein-coupled photoreceptor activity |
zgc:153154 | Small molecule binding in extracellular region | Small molecule binding activity |
cd74b | Ear development and neuromast development | MHC class II protein binding activity, cytokine receptor activity, macrophage migration inhibitory factor binding activity |
zgc:153911 | T cell receptor signaling, cytokine production regulation | Signaling receptor binding activity in membrane |
pah | Cellular response to estrogen stimulus in structures incl. Digestive system, epidermis, eye, optic vesicle, and periderm | Phenylalanine 4-monooxygenase activity |
rasef | GDP and GTP binding in cytosol and perinuclear region of cytoplasm | Enables GDP and GTP binding activity |
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Thompson, E.; Hensley, J.; Taylor, R.S. Effect of High Glucose on Embryological Development of Zebrafish, Brachyodanio, Rerio through Wnt Pathway. Int. J. Mol. Sci. 2024, 25, 9443. https://doi.org/10.3390/ijms25179443
Thompson E, Hensley J, Taylor RS. Effect of High Glucose on Embryological Development of Zebrafish, Brachyodanio, Rerio through Wnt Pathway. International Journal of Molecular Sciences. 2024; 25(17):9443. https://doi.org/10.3390/ijms25179443
Chicago/Turabian StyleThompson, Ebony, Justin Hensley, and Renfang Song Taylor. 2024. "Effect of High Glucose on Embryological Development of Zebrafish, Brachyodanio, Rerio through Wnt Pathway" International Journal of Molecular Sciences 25, no. 17: 9443. https://doi.org/10.3390/ijms25179443